从牛的选择特征到小鼠的功能验证:HSPA12B负面调节脂肪色和发热
Yaping Gao1,2, Jinpeng Wang1,2, Qiang Jiang1,2
1Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Journal of animal science
|January 30, 2026
概括
热冲击蛋白A12B (HSPA12B) 通过控制脂肪组织色调节冷适应. 它的抑制促进了热生成,为肥胖和糖尿病提供了潜在的治疗点.
科学领域:
- 动物遗传学和动物基因组学
- 分子生物学分子生物学
- 生理学 生理学 生理学
背景情况:
- 牛种通过选择表现出适应不同气候的能力.
- 动物适应寒冷的分子基础,特别是涉及脂肪组织色的分子基础,尚不清楚.
- 热冲击蛋白A12B (HSPA12B) 是适应寒冷的候选基因.
研究的目的:
- 研究HSPA12B在牛和小鼠寒冷适应中的作用.
- 确定HSPA12B影响脂肪组织色和热生成的分子机制.
- 探索HSPA12B作为代谢障碍治疗点的潜力.
主要方法:
- 对来自中国牛品种的SNP基因定型数据的分析.
- 在牛和小鼠脂肪组织中进行基因表达分析 (西方斑点,qRT-PCR).
- 产生和分析Hspa12b淘汰赛小鼠模型.
- 蛋白质与蛋白质相互作用的预测.
- 在小鼠中进行葡萄糖和胰岛素耐受性测试.
主要成果:
- 在HSPA12B中,在适应寒冷的牛品种中发现了积极选择的证据.
- 在寒冷暴露时,HSPA12B的表达在脂肪组织中被上调.
- 通过对Elovl3和Ucp1.1进行上调调节,Hspa12b的删除增强了脂肪色和热生成.
- Hspa12b淘汰赛小鼠表现出改善的葡萄糖代谢和胰岛素敏感性.
- HSPA12B与ELOVL3相互作用,并负面调节脂肪色.
结论:
- HSPA12B作为脂肪组织色和热生成的负调节剂,由ELOVL3.3调节.
- 低温激活HSPA12B作为一个恒温机制,以防止过度脂肪色.
- 研究结果提供了对寒冷适应机制的见解,并建议HSPA12B作为肥胖和糖尿病治疗的目标.
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