在Physcomitrella中的拉巴胺素 (TOR) 标志目标的功能性表征
Elie Saliba1, Sebastian N W Hoernstein2, Nico van Gessel1
1Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestr. 1, 79104, Freiburg, Germany.
Plant cell reports
|January 30, 2026
概括
保守的目标拉帕米辛 (TOR) 激酶调节了模型Physcomitrella的生长,叶绿体发育和蛋白质合成. 抑制TOR信号传递会影响细胞循环的进展和光合作用,强调其在植物发育中的关键作用.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 拉帕素 (TOR) 激酶的点是真核生物中关键的信号中心,调节生长和发育.
- 在许多真核生物中,TOR信号得到了很好的研究,但在非血管植物中,如模型Physcomitrella (Physcomitrium patens) 等,人们对其理解较少.
- 了解Physcomitrella中的TOR信号提供了关于植物生理学,发育和进化的见解.
研究的目的:
- 调查TOR信号传递在Physcomitrella的生长和发育中的作用.
- 识别和描述Physcomitrella中TOR信号通路的关键组成部分.
- 阐明抑制TOR信号传递对Physcomitrella各种细胞过程的影响.
主要方法:
- 使用拉巴胺素和特定抑制剂AZD8055.5,抑制TOR信号传递.
- 过度表达Physcomitrella12-kDaFK506结合蛋白 (FKBP12),以评估对拉帕米辛的敏感性.
- 对TOR复合1 (TORC1) 组件 (PpTOR,PpLST8,PpRAPTOR) 的识别和表征以及条件突变的产生.
- 使用LC-MS/MS.对核糖体蛋白RPS6的TOR依赖酸化的分析.
主要成果:
- 通过AZD8055抑制TOR信号传递,或通过过度表达FKBP12,显著抑制了质子体的生长.
- 阻断TOR导致性效应,包括细胞循环进展延迟,化,光合作用减少和蛋白质含量改变.
- 确定了TORC1的关键组成部分 (PpTOR,PpLST8,PpRAPTOR),它们各自的突变显示出生长受损;PpLST8功能上取代了它的酵母同类.
- 证实了RPS6的TOR-依赖酸化,验证了PpTOR激酶活性.
结论:
- 进化保守的TOR激酶在Physcomitrella中积极控制生长,发育,叶绿体功能,蛋白质合成和细胞周期进展.
- 在这种模型中,TOR信号对于正常发育和生理功能至关重要.
- 对TORC1信号集成在Physcomitrella中的进一步研究可以阐明保存的植物信号机制.
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