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超越FERONIA:基因表达简介揭示了细胞壁在pectin含量不同的组织中malectin/malectin-like蛋白的功能多样化
N Syrchina1, N Mokshina1, N Petrova1
1Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russia.
雄性素和雄性素类蛋白 (M/MLPs) 是植物细胞壁的关键. 这项研究揭示了与pectin含量和细胞扩张相关的明显的M/MLP基因表达模式,这表明细胞壁传感中的专门功能.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 雄性菌素和雄性菌素类蛋白 (M/MLPs) 构成一个大型的植物菌素家族,其碳水化合物特异性在很大程度上未知.
- 已知M/MLP的FERONIA是一种类似受体的激酶,已证实可以结合pectins,突出显示M/MLPs在细胞壁相互作用中的作用.
研究的目的:
- 在植物细胞壁组成的背景下,研究M/MLP基因的功能专业化.
- 分析M/MLP基因的表达特征,与涉及不同植物物种细胞壁多糖生物合成的基因相关.
主要方法:
- 从Arabidopsis thaliana,玉米,菜和亚麻中转录组数据分析,其中的pectin含量各不相同.
- 识别和修改M/MLP基因并分析它们的表达模式.
- 对M/MLP基因与负责pectin,xyloglucan和纤维素生物合成的糖系转移酶 (GT) 基因的同时表达分析.
主要成果:
- 显著的M/MLP基因组在细胞扩张过程中,在富含pectin的组织中,以及在壁壁加厚过程中被上调,显示单种和双种植物之间部分保存.
- 在菜中观察到M/MLP基因和pectin生物合成GT之间强烈的共同表达.
- 在所有样本中,M/MLP基因与亚麻纤维中的pectin GTs的相关性很小,与西洛格卢干和纤维素生物合成GTs的负相关性.
结论:
- 有证据表明,在M/MLP家族中存在显著的功能专业化.
- 特别的M/MLP基因被突出显示为未来对pectin识别和植物细胞壁传感机制的研究的候选者.
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