在有氧条件下,Candida glycerinogenes的快速酒精发酵
Mengying Wang1,2, Xinke Wang1,2, Dongqi Jiang1,2
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Preparative biochemistry & biotechnology
|January 30, 2026
概括
在有氧条件下,与Saccharomyces cerevisiae相比,Candida glycerinogenes表现出优越的糖代谢和乙醇生产. 它的糖解酶在高ATP水平下保持活跃,推动快速发酵.
科学领域:
- 生物技术是生物技术.
- 微生物生理学 微生物生理学
- 工业发酵 工业发酵是什么
背景情况:
- 红纤维素解液的毒性阻碍了酒精发酵.
- 升高的溶解氧增加了微生物耐受性和发酵效率.
研究的目的:
- 在有氧条件下阐明Candida glycerinogenes的快速糖代谢机制.
- 将碳流量分配和糖代谢率与Saccharomyces cerevisiae进行比较.
- 研究NAD (H) 和ATP调节在快速发酵中的作用.
主要方法:
- 在C. glycerinogenes中检查了碳流量分配和糖代谢率.
- 评估了糖解酶活性和ATP抑制的敏感性.
- 在S. cerevisiae W303.3.中执行了PFK1基因过度表达.
主要成果:
- 而C. glycerinogenes的糖解率和乙醇生产率比S. cerevisiae更高.
- 在高ATP水平下,C. glycerinogenes保持了高的糖解酶活性,并增加了NADH/NAD+比率.
- 在S. cerevisiae中过度表达PFK1增加了14.48%的葡萄糖消耗率.
结论:
- 高糖解酶活性和ATP不敏感性是C. glycerinogenes快速发酵的关键.
- 为了理解快速发酵,NAD (H) 和ATP调节机制至关重要.
- 结果为优化乙醇和其他糖解产品产量提供了洞察力.
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