转录基因组分析表明,牛群中对Pasteurella multocida突变性感染的基因反应各异
Hao Ma1, Fred M Tatum1, Robert E Briggs1
1Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, United States of America.
PloS one
|January 30, 2026
概括
三种Pasteurella multocida突变物被评估为抗牛呼吸道疾病的活疫苗. fhaB2突变显示出有希望的结果,提供了减少肺病变和殖民化的保护.
科学领域:
- 兽医免疫学和疫苗学 兽医免疫学和疫苗学
- 细菌病原和宿主-病原体相互作用.
- 转录学和分子生物学在动物健康中的应用.
背景情况:
- 牛呼吸道疾病 (BRD) 显著影响牛的健康和生产力,需要有效的控制策略.
- 活体减弱疫苗提供了一个有希望的方法来诱导强大的,持久的免疫力对BRD与最小的不良影响.
- 帕斯托雷拉多种菌是BRD的关键病原体,因此疫苗开发对于动物福利和经济原因至关重要.
研究的目的:
- 评估三种Pasteurella multocida突变体 (fhaB2,hyaE,nanP) 作为潜在的活体疫苗候选人,以对抗BRD.
- 通过临床挑战研究和转录组分析来评估这些突变的保护效果.
- 使用转录基因数据识别潜在的宿主反应和疫苗疗效的分子机制.
主要方法:
- 通过破坏 fhaB2,hyaE 和 nanP 基因在血清组 3 菌株 (P1062) 中生成三种 P. multocida 突变物.
- 通过挑战研究对突变疗效的临床评估,评估肺病变和殖民率.
- 血液和肝脏组织的转录组分析以确定差异表达的蛋白质编码转录 (DET),微RNA (DEmiRNA) 和长非编码RNA (DElncRNA).
主要成果:
- 所有三种P. multocida突变都对挑战提供了保护,与假装组 (22.4%) 相比,显著减少了肺病变 (4.7-6.2%).
- 转录组分析揭示了血液 (807) 和肝脏 (6473) 中大量的DET,以及差异表达的非编码RNA.
- 这种 fhaB2 突变体显示出有前途的疫苗潜力,通过减少肺病变和殖民来证明这一点,并得到了转录组数据的支持,这些数据显示了不同的宿主反应.
结论:
- 牛群中,P. multocida的 fhaB2,hyaE 和 nanP 突变提供了对 BRD 挑战的保护.
- 转录组分析提供了对宿主对疫苗接种和感染的反应的见解,突出了与信号和感官感知相关的途径.
- 鉴于 fhaB2 突变的有效性和有利的转录基因特征,它被认为是针对牛呼吸系统疾病的活体减弱疫苗的有希望的候选人.
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