适应性实验室进化和转录基因分析揭示了碳-代谢重编程,使Saccharomyces cerevisiae中的葡萄糖和树脂糖能够进行有氧共发酵
Lina Maria López-deÁvila1, Zulma Isabel Monsalve-Fonnegra2, Héctor Alejandro Rodríguez-Cabal3
1Grupo Biotransformación, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia.
PloS one
|January 30, 2026
概括
一种进化的Saccharomyces cerevisiae菌株 (F2C7A) 显示出改善了用于生物乙醇生产的氧化糖代谢. 这种工程酵母有效地共同发酵葡萄糖和西洛斯,减少西利副产品,并提高从林氏纤维素生物质中获得的乙醇产量.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 生物能源是生物能源.
背景情况:
- 麦芽菌 (Saccharomyces cerevisiae) 难以有效地代谢像西洛斯这样的纤维素糖,从而限制了生物乙醇的生产.
- 现有的重组菌株需要进一步优化,以实现葡萄糖和西洛斯的强有力的共发酵.
研究的目的:
- 开发一种改进的Saccharomyces cerevisiae菌株,以提高糖的利用率和混合糖的共同发酵.
- 为了研究与改进的基因酶代谢相关的代谢和转录基因变化,在工程菌株.
主要方法:
- 采用紫外线突变,原质细胞融合和适应性实验室进化来产生一个进化的S. cerevisiae菌株 (F2C7A).
- 评估合成介质中单一 (糖) 和混合糖 (葡萄糖和糖) 的应变性能.
- 进行转录基因分析以阐明代谢途径调节.
主要成果:
- 进化后的F2C7A菌株在72小时内消耗的量 (87.9%) 显著高于单独消耗的父菌株 (52.3%).
- 在混合糖条件下,F2C7A有效地消耗了葡萄糖和33%的氧化糖,产生乙醇 (0.45g/g),氧化醇积累最小.
- 转录组数据显示,关键的西洛代谢基因 (XYL1,XYL2),TCA循环和氧化酸化的上调调节,以及改变的代谢和糖载体表达.
结论:
- 结合古典和进化策略,可以有效地增强S. cerevisiae中的西洛代谢.
- 开发的菌株表现出改善的共同发酵能力和减少的西利醇形成,这对于高效的林氏纤维素生物乙醇生产至关重要.
- 这些发现为进一步改善工业生物乙醇应用的菌株提供了基础.
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