盒子-Behnken优化盐出辅助液体-液体提取与LC-MS/MS相结合,用于人类血中可持续的阿米苏普里德量化
Ahmed Serag1, Manal E Alosaimi2, Maram H Abduljabbar3
1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Nasr City 11751, Cairo, Egypt.
Journal of chromatography. A
|January 30, 2026
概括
一种新的可持续方法使用SALLE-LC-MS/MS精确量化了人体血中的阿米苏普里德. 这种方法是环保的,高效的,适合治疗药物监测.
科学领域:
- 分析化学 分析化学
- 药理动力学 药理动力学
- 绿色化学 绿色化学
背景情况:
- 准确量化阿米苏普利对于治疗药物监测至关重要.
- 现有的方法可能缺乏可持续性或效率.
研究的目的:
- 开发和验证一种可持续的化辅助液体-液体提取与液体染色学-并联质谱法 (SALLE-LC-MS/MS) 方法相结合的方法,用于在人体血中量化阿米苏普里德.
- 确保该方法遵守ICH M10指南,并展示绿色化学原则.
主要方法:
- 使用Box-Behnken设计,系统优化SALLE参数 (pH,酸体积,盐度,离心时间).
- 使用C18列,同位素化和多重反应监测进行LC-MS/MS分析.
- 验证线性,准确性,精度,稳定性和矩阵效应.
- 使用CaFRI,BAGI和RGB12框架进行可持续性评估.
主要成果:
- 优化条件产生了97.6%的提取回收.
- 经过验证的方法显示线性从2-1500 ng/mL高准确度和精度.
- 在志愿者中成功确定了药理动力学参数 (Cmax,tmax,t1/2).
- 可持续性指标证实了该方法的环保性和可行性.
结论:
- 开发的SALLE-LC-MS/MS方法是一种可持续,高效和合规的方法,用于阿米苏普里德的量化.
- 这种方法支持治疗药物监测,对环境产生最小的影响,并提高吞吐量.
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