在GIFT鱼 (Oreochromis niloticus) 中TRADD的分子表征
Youchuan Wei1, Honglian Tan1, Jiashu Li2
1China (Guangxi)-ASEAN Key Laboratory of Comprehensive Exploitation and Utilization of Aquatic Germplasm Resources, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquaculture genetic and breeding and Healthy Aquaculture of Guangxi Academy of Fishery Sciences, Nanning, 530021, China.
Developmental and comparative immunology
|January 30, 2026
概括
基因改进的养殖鱼 (GIFT) TRADD蛋白质在抗病毒防御中起着关键作用. 这项研究表明,GIFT TRADD增强了对鱼湖病毒 (TiLV) 和细菌感染的免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 水产养殖是水产养殖的一种方式.
背景情况:
- 瘤亡因子受体1型关联死亡域蛋白 (TRADD) 在TNF信号传递中至关重要.
- 与哺乳动物相比,鱼类的功能尚不清楚.
研究的目的:
- 在基因改进的养殖鱼 (GIFT,Oreochromis niloticus) 中描述TRADD基因.
- 调查礼物贸易在免疫反应和抗病毒防御中的作用.
主要方法:
- 在 GIFT 中进行 TRADD 基因表征.
- 定量PCR (qPCR) 用于分析各种组织和刺激后的基因表达.
- 基于细胞的测试,以评估 GIFT TRADD 过度表达对病毒感染和免疫基因表达的影响.
主要成果:
- GIFT TRADD编码了一个291氨基酸蛋白质,具有N端和C端死亡域.
- 礼物贸易在LPS,多I:C刺激和TiLV感染后在脏中得到广泛表达和上调.
- 过度表达GIFT TRADD抑制了TiLV-S10的表达,并促进了MDA5,Mx1,NF-κB和IRF3在鱼脑细胞中的表达.
结论:
- 礼品贸易表现出显著的抗病毒和免疫调节功能.
- 这项研究为GIFT中的TNF信号通路及其在免疫中的作用提供了基础的见解.
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