有证据表明,在海洋鱼 (Oryzias melastigma) 中,免疫发育的潜在关键窗口存在
Elizabeth DiBona1, Yuan Lu2, Justin Elliot3
1Department of Life Sciences, Texas A&M University-Corpus Christi, Corpus Christi, TX, USA.
概括
早期发育期间的环境暴露会影响长期的免疫健康. 这项研究使用海洋梅达卡鱼来识别先天性免疫系统成熟的敏感窗口,并评估17α-乙inylestradiol (EE2) 等内分泌干扰物的风险.
科学领域:
- 发育生物学是发展生物学.
- 免疫学 免疫学 免疫学
- 环境毒理学环境毒理学
背景情况:
- 健康和疾病的发育起源 (DOHaD) 假设将早期暴露与以后的病理联系起来.
- 对于脊椎动物的先天免疫成熟的敏感发育窗口还没有很好地定义.
- 海洋梅达卡 (Oryzias melastigma) 被提出作为建立先天免疫发育时间表的模型.
研究的目的:
- 定义一个分子时间表,用于天生的免疫系统在海洋中成熟.
- 确定免疫发育和对环境暴露的脆弱性的关键窗口.
- 评估17α-乙inylestradiol (EE2) 对免疫发育和功能的影响.
主要方法:
- 评估了生物体在不同发育阶段对细菌感染的抵抗力.
- 在整个发育过程中监测免疫基因表达标记物.
- 在特定的发育时期 (受精后7-11天,化后3-5天,化后12-19天) 暴露于EE2的海洋medaka胚胎和幼虫,并受到细菌的挑战.
主要成果:
- 在胚胎和幼虫阶段确定了细菌感染脆弱性的明显窗口.
- 在胚胎 (7-11 dpf) 和晚期幼虫 (12-19 dph) 期间暴露于EE2显著降低了病原体的耐药性.
- 暴露于EE2会改变与免疫相关的基因表达 (蛋白质合成,核糖体生物发生) 和改变能量/氨基酸代谢.
结论:
- 强调确定敏感的发育时期对于评估环境风险至关重要.
- 支持使用海洋medaka作为研究发育性免疫毒性的相关模型.
- 表明在特定的窗口中暴露于EE2会影响先天免疫能力和新陈代谢.
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