远端设计改善了Nitrosospira产生的III型铁酶的热稳定性和酶活性
Mo Han1, Mengli Li1, Ruoyu Jia1
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, 214122, China.
International journal of biological macromolecules
|January 30, 2026
概括
研究人员为改善工业应用设计了一种微生物铁酶 (TYR) 酶. 蛋白质工程显著提高了它的活性和热稳定性,展示了用于酶优化的新型远程设计策略.
科学领域:
- 生物化学 生物化学
- 酵素工程是什么? 酶工程是什么
- 生物技术是生物技术.
背景情况:
- 铁酶 (TYR) 是一种重要的含铜氧化酶,在黑色素合成中,在各种生物体中发现.
- 目前TYR的工业应用受到低催化效率和稳定性差的限制.
研究的目的:
- 为生物技术应用识别和设计一种高度活跃和稳定的微生物铁酶.
- 探索一种合理的远程设计策略对酶优化的有效性.
主要方法:
- 系统的基因组挖掘发现了来自Nitrosospira (Sp2) 的III型TYR.
- 构建了C端截断突变体,并采用了与蛋白质工程计算模拟的远程设计策略.
- 利用AlphaFold 3进行结构预测和分子对接来分析酶特性.
主要成果:
- 一种被截断的突变,TYR-Sp2-276,显示酶活性增加了15%.
- 一种组合突变 (TYR-Sp2-276-G73A/M106D/Q152A/M231P) 显示酶活性增强2.37倍 (654个单位/毫克).
- 工程酶显示化温度增加了4.59°C,kcat值提高了2.55倍.
结论:
- 理性远程设计是增强酶活性和热稳定的有效策略.
- 工程化微生物铁酶显示了工业应用的巨大潜力.
- 这项研究为设计其他工业相关的微生物酶提供了宝贵的见解.
相关概念视频
Types of Functions III
263
Logarithmic and piecewise functions play central roles in mathematical modeling, particularly when capturing nonlinear or segmented behaviors in real-world phenomena. Although these functions differ fundamentally in structure and application, both serve to represent complex relationships in simplified mathematical terms.A logarithmic function is defined as the inverse of an exponential function, expressed as These functions grow quickly for small values of x but slow down as x increases,...
263
Enzymes and Activation Energy
23.6K
The activation energy (or free energy of activation), abbreviated as Ea, is the small amount of energy input necessary for all chemical reactions to occur. During chemical reactions, certain chemical bonds break, and new ones form. For example, when a glucose molecule breaks down, bonds between the molecule's carbon atoms break. Since these are energy-storing bonds, they release energy when broken. However, the molecule must be somewhat contorted to get into a state that allows the bonds to...
23.6K
Enzymes
94.7K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.7K
ATP Driven Pumps III: V-type Pumps
4.8K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
4.8K
Types of Reports III: Telephone and Verbal Reports
1.0K
Telephone and Verbal Reports in healthcare settings are two communication methods for conveying therapeutic instructions from healthcare providers to nurses or other healthcare staff.
Here's an overview of each type:
Telephone Orders
Here's an overview of each type:
Telephone Orders
1.0K
Structure-Activity Relationships and Drug Design
1.8K
Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
1.8K


