从Morchella esculenta树枝提取蛋白质方法的比较研究:物理化学,营养,消化和功能性质的全面分析
Xianzhi Li1, Minyu Li1, Emad Hamady Khedr2
1State Key Laboratory for the Development and Utilization of Forest Food Resources, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.
International journal of biological macromolecules
|January 30, 2026
概括
亚临界水提取 (SWE) 是从M. esculenta stipe.中分离蛋白质的最有效方法. 这种可持续的方法产生了高质量的蛋白质,具有增强的功能和营养特性.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 摩切拉 (Morchella esculenta stipe) 是一种有价值的蛋白质的潜在来源.
- 优化蛋白质提取方法对于利用这种资源至关重要.
研究的目的:
- 为了比较四种蛋白质提取方法:性提取 (AE),超声波辅助性提取 (UAE),亚临界水提取 (SWE) 和深度性溶剂提取 (DES).
- 评估这些方法对M. esculenta stipe蛋白质分离物 (PIM) 的提取效率,结构特征,功能性质和营养质量的影响.
主要方法:
- 使用AE,UAE,SWE和DES提取蛋白质.
- 提取的蛋白质被分析为产量,可溶性,抗氧化活性,体外消化性,结构特征 (FTIR,SEM),乳化能力,水容量 (WHC),油容量 (OHC),氨基酸概况和必需氨基酸指数 (EAAI).
主要成果:
- 亚临界水提取 (SWE) 产生了最高的蛋白质提取率 (25.87%).
- 来自SWE的蛋白质 (SW-P) 显示出更高的溶解度 (53.7%),抗氧化活性和体外消化能力 (99.07%).
- 由于有多孔的微观结构,SW-P显示了增强的乳化能力,WHC和OHC. 它还具有最高的总氨基酸含量 (82.13 g/100 g),EAAI (52.75%),和必需氨基酸比例 (42.73%).
结论:
- 亚临界水提取 (SWE) 是一种高效和可持续的方法,用于从Morchella esculenta stipes中回收高质量的蛋白质.
- 在蛋白质产量,功能性质和营养价值方面,SWE比AE,UAE和DES具有显著的优势.
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