相关实验视频
Updated: Feb 1, 2026

21:24
Methylated DNA Immunoprecipitation
Published on: January 2, 2009
24.2K
铜中的DNA甲基化媒介变化 (I/II) 降氧平衡是诱导的神经毒性的基础
1School of Energy and Environment and State Key Laboratory of Marine Environmental Health, City University of Hong Kong, Kowloon, Hong Kong, China; Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China.
Environmental pollution (Barking, Essex : 1987)
|January 30, 2026
概括
暴露于会通过改变DNA甲基化和蛋白质表达,破坏金属平衡和细胞能量来损害大脑健康. 这种神经毒性影响神经系统发育和神经退行性疾病至关重要的途径.
科学领域:
- 神经科学是一个神经科学.
- 毒理学 毒理学 毒理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- (Pb) 是一种神经毒素,它破坏了大脑中的金属平衡.
- Pb对铜 (Cu) 反氧化状态的分子影响及其表观遗传效应尚未完全理解.
研究的目的:
- 研究SH-SY5Y细胞中Pb诱导的神经毒性的分子机制.
- 分析Pb暴露引起的综合表观基因和蛋白质基因变化.
主要方法:
- 综合表观基因组学 (DNA甲基化分析) 和蛋白质组学 (蛋白质表达分析).
- 对差异甲基化区域 (DMR) 和差异表达蛋白质 (DEP) 的分析.
主要成果:
- 确定了141,357个DMRs,与与金属离子结合和神经系统发育相关的基因有显著的丰富.
- 检测到740个DEP,在线粒体中丰富,影响氧化酸化和离子运输.
- 观察到Cu氧化还原状态和谷氨 (GSH) 活性的破坏,表明金属稳态受损和氧化失衡.
结论:
- 暴露于Pb显著改变神经元细胞中的表观遗传和蛋白质基因格局.
- Pb诱导的神经毒性涉及到细胞能量代谢,金属离子动态和氧化平衡的破坏.
- 表观遗传和蛋白质变化提供了对Pb对神经元健康和神经退行性疾病途径的影响的全面了解.
相关概念视频
Balancing Redox Equations
62.1K
Electrochemistry is the science involved in the interconversion of electrical and chemical reactions. Such reactions are called reduction-oxidation, or redox reactions. These important reactions are defined by changes in oxidation states for one or more reactant elements and include a subset of reactions involving the transfer of electrons between reactant species. Electrochemistry as a field has evolved to yield sufficient insights on the fundamental principles of redox chemistry and multiple...
62.1K
Redox Reactions
58.7K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
58.7K
Redox Reactions
1.0K
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
1.0K
Dynamic Equilibrium
62.6K
A reversible chemical reaction represents a chemical process that proceeds in both forward (left to right) and reverse (right to left) directions. When the rates of the forward and reverse reactions are equal, the concentrations of the reactant and product species remain constant over time and the system is at equilibrium. A special double arrow is used to emphasize the reversible nature of the reaction. The relative concentrations of reactants and products in equilibrium systems vary greatly;...
62.6K
Free Energy and Equilibrium
27.2K
The free energy change for a process may be viewed as a measure of its driving force. A negative value for ΔG represents a driving force for the process in the forward direction, while a positive value represents a driving force for the process in the reverse direction. When ΔGrxn is zero, the forward and reverse driving forces are equal, and the process occurs in both directions at the same rate (the system is at equilibrium).
Recall that Q is the numerical value of the mass action...
Recall that Q is the numerical value of the mass action...
27.2K
Calculating the Equilibrium Constant
38.0K
The equilibrium constant for a reaction is calculated from the equilibrium concentrations (or pressures) of its reactants and products. If these concentrations are known, the calculation simply involves their substitution into the Kc expression.
For example, gaseous nitrogen dioxide forms dinitrogen tetroxide according to this equation:
For example, gaseous nitrogen dioxide forms dinitrogen tetroxide according to this equation:
38.0K

