血淋巴采样方法的比较研究:养殖Crassostrea corteziensis (Bivalvia:) 的血细胞特征的每月变化
Miguel Ángel Hurtado-Oliva1, Elena Palacios-Mechetnov2
1Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa, Paseo Claussen s/n, 82000, Mazatlán, Sinaloa, México.
在 (C. corteziensis) 的实地与实验室血淋巴抽取没有显示免疫细胞数量或功能上的差异. 季节性环境因素显著影响了的免疫参数,植物浮游生物的存在与血细胞数量增加和氧化能力相关.
科学领域:
- 海洋生物学 海洋生物学
- 免疫学 免疫学 免疫学
- 水产养殖是水产养殖的一种方式.
背景情况:
- 两性软体动物的免疫反应对温度,盐度和食物可用性等环境条件敏感.
- 水产养殖中的处理程序,包括储存和运输,可以改变免疫细胞的特征.
- 了解这些影响对于准确评估健康和免疫状况至关重要.
研究的目的:
- 评估血淋巴抽取方法 (现场与实验室) 对免疫细胞形态和功能的影响.
- 分析C. corteziensis. 的血细胞特征的每月变化.
- 为了将免疫参数与环境因素 (海水温度,盐度,塞斯顿,叶绿素a) 相关联.
主要方法:
- 血淋巴从C. corteziensis直接在野外收集,并在经过2小时的陆地运输到实验室后.
- 评估的全血细胞计数 (THC),微分血细胞计数 (DHC),血细胞死亡率 (HM) 和离子超氧化物生产 (ASO/血细胞).
- 每个月监测海水的物理化学参数:SST,盐度,pH,塞斯和叶绿素-a (Chl-a).
主要成果:
- 在实地和实验室处理的血淋巴样本之间,没有发现THC,DHC,HM或ASO/血细胞的显著差异.
- 根据采样日期,免疫参数有显著的变化,THC和氨酸细胞数量与Chl-a (植物浮游生物的可用性) 有正相关.
- 血细胞的氧化能力随着的状况和浮游植物的可用性而增加,这可能与dinoflagellate消费有关.
结论:
- 血淋巴抽取方法 (现场与实验室) 不会影响C. corteziensis.中的关键血细胞免疫参数.
- 季节性环境变化,特别是植物浮游生物的存在,是的免疫状况的主要驱动因素.
- 现场血淋巴采集有利于仅需要免疫细胞和血进行分析,避免运输压力.
更多相关视频
08:42Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers
Published on: March 17, 2015
10:59Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies
Published on: June 1, 2019
相关概念视频
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
What is Variation?
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
Sampling Methods: Sample Types
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
Characteristics of Life
Variation
When independent and dependent variables are plotted on a scatter plot, the slope of a line is a value that describes the rate of change between the two...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
