仪器仪表和NaOCl度对微生物DNA负载的影响,使用声波激活装置
Matthew Ellis1, Ronald Ordinola-Zapata2, Marco A Versiani3
1Division of Endodontics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN.
Journal of endodontics
|January 30, 2026
概括
带有声波激活的根运河仪器显著降低了下牙中的细菌DNA负载. 在使用声波激活时,灌剂的类型和度对细菌减少的影响最小.
科学领域:
- 牙周内科医院 牙周内科医院 牙周内科医院
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 细菌污染是内牙失败的主要原因.
- 根管的有效消毒对于成功治疗至关重要.
- 声波激活可以提高灌剂的透率和效率.
研究的目的:
- 量化细菌DNA负载减小在下牙的 mesial 根通道的量化.
- 评估仪器仪表,灌剂类型和声波激活对细菌DNA减少的影响.
- 为了确定仪器仪表和灌剂类型在减少细菌负载方面的相互作用.
主要方法:
- 人类下牙被斑块污染,并通过声波激活进行根管制备.
- 测试了三组灌剂:7.55%的NaOCl,1.88%的NaOCl和无菌盐水.
- 细菌DNA在基线使用定量聚合酶链反应 (qPCR) 量化,在初始制备后 (尺寸25/.04) 和扩大后 (尺寸30/.04).
主要成果:
- 使用声波激活仪器显著降低了细菌DNA负载 (P < 0.0001).
- 大多数细菌减少发生在初始制备过程中,以大小为25/.04.
- 不同的灌剂类型或度之间在与声波激活相结合时,没有观察到细菌DNA减少的显著差异.
结论:
- 根通道仪器与声波激活相结合,是细菌DNA负载减轻的主要驱动因素.
- 在使用声波激活时,灌剂的类型和度在细菌减少中起不大作用.
- 声波激活显著提高了根管制剂的抗菌疗效.
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