相关实验视频

Updated: Feb 1, 2026

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
11:42

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Published on: November 4, 2019

6.5K

在大肠杆菌中功能性人类基本纤维细胞生长因子2的过度表达

Tamilvendan Manavalan1, Jiawu Bi2, Khongsay Naulchan3

  • 1Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, Singapore; Centre for Precision Fermentation and Sustainability, Illinois Advanced Research Center at Singapore, Singapore 138602.

Protein expression and purification
|January 30, 2026
PubMed
概括

研究人员开发了一种具有成本效益的方法,用于在大肠杆菌中产生人类基本纤维细胞生长因子2 (hbFGF2). 这种高产量的活性hbFGF2的生产对于降低培养肉介质成本和提高可扩展性至关重要.

关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.这是一种HepG2型肝炎.在 Ph9F-1x 的情况下.蛋白质表达方式 蛋白质表达方式hbFGF2-G3 这是一个很好的例子.

更多相关视频

The Multifaceted Benefits of Protein Co-expression in Escherichia coli12:48

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

12.5K
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K

相关概念视频

Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
3.9K
Factors Influencing Microbial Growth: pH01:29

Factors Influencing Microbial Growth: pH

Microorganisms are classified as acidophiles, neutrophiles, or alkaliphiles based on their pH growth preferences, reflecting their adaptations to specific environments. Maintaining a stable intracellular pH is critical for macromolecular stability and enzymatic activity, which can be challenged by external pH variations.Neutrophiles, such as Escherichia coli, grow optimally between pH 5.5 and 8.0. These microorganisms inhabit neutral or slightly acidic environments and employ mechanisms like...
1.2K
Maslow's Theory of Basic Human Needs01:28

Maslow's Theory of Basic Human Needs

Maslow's hierarchy is described with the help of a pyramidal shape. The most fundamental needs, physiological needs, are at the bottom of the pyramid.
Physiological needs such as hunger, thirst, sex, physical comfort, and survival are at the bottom of the pyramid. These are the components that are necessary to sustain life. Once the first level of needs has been met, the second level arises.
Safety needs include stability and predictability. Protection and freedom from danger are all a part...
3.2K
Factors Influencing Microbial Growth: Temperature01:27

Factors Influencing Microbial Growth: Temperature

Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
1.2K
Factors Influencing Microbial Growth: Osmolarity01:28

Factors Influencing Microbial Growth: Osmolarity

Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...
870
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.7K