烯胺在卵细胞成熟过程中破坏介质G2/M过渡和SAC活性
Shanshan Chen1,2, Peng-Xia Wang2, Yan-Ting Wang1
1Department of Reproduction, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, 213300, China.
Biology direct
|January 31, 2026
概括
烯胺暴露破坏了小鼠卵细胞的半转化,导致DNA损伤和异常的微管附着物. 这种化学物质干扰细胞循环的进展,影响生殖囊泡的分解和极体挤出.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 烯胺是一种在食品和工业过程中发现的有毒物质.
- 以前的研究将烯胺与线粒体功能障碍和卵细胞的亡联系起来.
研究的目的:
- 为了研究烯胺对小鼠卵子细胞减量和细胞循环进展的影响.
主要方法:
- 检查了生殖囊泡 (GV) 的分解和极体挤出.
- 使用 γ-H2A.X 表达式评估DNA损伤.
- 分析了细胞循环调节剂,如环素B1和CDK1.
- 研究了动态管-微管附着部件和螺旋组装检查点 (SAC) 组件 (BubR1,Bub3).
- 通过氨酸乙化 (HDAC6,NAT10) 和冷处理评估了微管稳定性.
主要成果:
- 烯胺诱导DNA损伤和GV分解和极体挤出中的缺陷.
- 改变了循环B1,CDK1,CHK2,BubR1和Bub3的表达,表明细胞循环中断.
- 观察到异常的kinetochore-microtubule附件和受损的SAC激活.
- 降低管乙化减少了微管的稳定性.
结论:
- 暴露在烯胺中会破坏小鼠卵细胞的半衰期.
- 细胞循环进展通过DNA损伤介导的成熟促进因子 (MPF) 活性和氨酸乙介导的SAC激活受损.
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