后翻译修饰物的水平转移为保存的翻译因子带来了进化机会和挑战
Tess E Brewer1,2, Pavel Kielkowski3, Jingzhi Stritzel4
1Faculty of Biology, Microbiology, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany. tess@tess-brewer.com.
BMC biology
|January 31, 2026
概括
主体细胞环境显著影响横向转移的基因的功能,如翻译延长因子P (EF-P). 一些EF-P变体在没有修改的情况下起作用,而另一些则受到非目标后翻译修改 (PTM) 的阻碍.
科学领域:
- 微生物进化过程中的微生物.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 横向基因转移 (HGT) 驱动微生物进化,但宿主环境对转移的基因功能的影响尚不清楚.
- 通过HGT.调查宿主对转化后修改 (PTM) 机器采购的影响.
- 作为模型系统,使用Escherichia coli中的多种物种转化延长因子P (EF-P) 的异构表达.
研究的目的:
- 调查主机背景如何影响水平获得的PTM机器的集成和功能.
- 为了确定宿主细胞环境对EF-P变体的功能以及各种PTM要求的影响.
主要方法:
- 来自E. coli中的各种物种EF-P的异质表达.
- 在大肠杆菌EF-P淘汰菌株中评估EF-P功能.
- 分析宿主特异性PTM酶 (如EarP) 对异质EF-P的影响.
主要成果:
- 来自D. radiodurans,G. ferrihydriticus和N. communis的EF-P补充了E. coli没有PTM的EF-P淘汰.
- 来自M.prima的EF-P经过了EarP的有害的非位拉姆诺基化.
- 耳P介导的拉姆诺基化不影响EF-P样蛋白EfpL的功能.
结论:
- 通过HGT获得的PTM系统可以对宿主蛋白产生可变的影响.
- 目标外的PTM,如化,对EF-P变体产生不同的影响.
- 了解外来基因组语境中的基因整合和功能兼容性对于预测HGT结果至关重要.
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