开发一种生物传感器,以光谱测定人工唾液中的L-乳酸盐
Rehab E Bayoumy1, Nariman A El-Ragehy2, Nagiba Y Hassan2
1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, Cairo, 11562, Egypt. rehab.essam@pharma.cu.edu.eg.
BMC chemistry
|January 31, 2026
概括
一个新的,非侵入性的色度生物传感器准确地检测L-乳酸盐使用铁 (III) 离子而不是酶. 这种敏感的方法为人工唾液样本的临床L-乳酸盐分析提供了一个有前途的工具.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- L-乳酸盐是临床诊断中的关键生物标志物.
- 传统的L-乳酸盐测定方法通常依赖于酶分析,这些分析可能是复杂的或昂贵的.
- 对于L-乳酸盐分析,需要灵敏,非侵入性和经济高效的生物传感器.
研究的目的:
- 开发一种新的,非侵入性的色度生物传感器,用于准确确定L-乳酸盐.
- 为了研究铁(III) 离子在L-乳酸盐检测中作为过氧化酶模仿剂的使用.
- 通过人工唾液样本验证生物传感器的性能.
主要方法:
- 设计了一个色度生物传感器,利用乳酸氧化酶 (LOx) 释放过氧化 (H2O2).
- 铁(III) 离子 (Fe3+) 催化了H2O2和3,3',5,5'-四甲基 (TMB) 基底之间的反应.
- 颜色变化在652nm时通过光谱测量,反应在37°C和30°C时得到优化.
主要成果:
- 生物传感器显示L-乳酸盐的线性反应在5μM20μM的范围内.
- 达到了1.278μM的低检测极限 (LOD),表明高灵敏度.
- 该方法在应用于人工唾液样本时显示了可重现的结果和令人满意的性能.
结论:
- 铁3+离子有效地模仿的过氧化酶活性,用于对颜色进行L-乳酸盐的测定.
- 开发的生物传感器是敏感的,非侵入性的,适合临床应用.
- 这种方法为L-乳酸盐分析提供了一个有希望的替代方案,可能降低成本和复杂性.
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