在 Bactericera cockerelli (Hemiptera: Triozidae) 中通过沉默编码全球生物过程所必需的质子的单个或堆叠基因进行RNA干扰
Mosharrof Mondal1, Jorge R Paredes-Montero1,2,3, Esmaeil Saberi4,5
1School of Plant Sciences, The University of Arizona, Tucson, AZ, USA.
Journal of economic entomology
|January 31, 2026
概括
针对真空ATPase (V-ATPase) 基因的堆叠双链RNAs (dsRNAs) 显示出通过增加死亡率和减少卵位,具有显著的潜力作为针对土豆虫的生物杀虫剂, Bactericera cockerelli.
科学领域:
- 农业昆虫学 农业昆虫学
- 分子昆虫学分子昆虫学
- RNA干扰 (RNAi) 技术是一种技术.
背景情况:
- "候选人"是Liberibacter spp. 的一种. 是重要的作物害虫,由虫传播.
- RNAi已经显示出对害虫控制的前景,一些昆虫物种容易受到影响.
- 空腔ATPase (V-ATPase) 对昆虫的生理过程至关重要.
研究的目的:
- 为了评估四个基本的V-ATPase亚单元基因 (A,B,D,E) 在马psyllid, Bactericera cockerelli. 中的淘汰效果.
- 评估针对V-ATPase基因的RNAi作为针对这种昆虫载体的生物杀虫剂的潜力.
主要方法:
- 马psyllids (成年人和第三阶段) 被暴露在单个或堆叠的双链RNA (dsRNAs) 中,准V-ATPase子单元48小时.
- 死亡率,基因淘汰效率和卵位减少被测量为摄入后访问期 (IAP).
- 单个和堆叠的dsRNA治疗方法之间的比较,以及不同种类动物生命阶段之间的比较.
主要成果:
- 与对照组相比,堆叠的dsRNAs显著增加了成人和第三阶段psyllids的死亡率.
- 接受治疗的雌性卵巢的排卵量减少了50%至70%,单个和堆叠的dsRNA之间没有显著差异.
- 基因沉默通常在比成年人更有效;堆叠的dsRNAs在生命阶段显示出相等或更大的死亡率.
结论:
- 用堆叠的dSRNA准必要的V-ATPase基因是开发有效的RNAi基生物杀虫剂的有希望的策略,用于对抗马psyllid.
- 这种方法通过同时抑制多个必需基因来提高RNAi的有效性.
- 对V-ATPase基因沉默的进一步研究可能会导致新的害虫管理解决方案.
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