胰岛素样生长因子-1 调节乳腺上皮细胞中血管内皮生长因子的合成和分泌
Asami Kawamura1, Kei-Ichi Nakajima1, Yuya Nagasawa2
1Department of Biochemistry, Asahikawa Medical University, 2-1-1-1 Midorigaoka Higashi, Asahikawa City, Hokkaido, Japan.
Bioscience, biotechnology, and biochemistry
|January 31, 2026
概括
胰岛素样生长因子1 (IGF1) 通过刺激血管内皮生长因子 (VEGF) 生产,促进乳腺发育. 这一过程涉及MEK-ERK1/2和PI3K-Akt信号通路,提高了哺乳效率.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 细胞信号传输 细胞信号传输
背景情况:
- 乳腺循环系统对于生长和哺乳至关重要,提供必要的氧气和营养.
- 胰岛素类生长因子1 (IGF1) 在乳腺发育中起着关键作用,但其在调节血管生成中的作用尚未完全理解.
研究的目的:
- 为了确定IGF1是否刺激乳腺上皮细胞中的血管内皮生长因子 (VEGF) 生产.
- 研究介导IGF1诱导的VEGF生产的信号通路.
主要方法:
- 在IGF1刺激后,在牛乳腺上皮和HC11细胞中评估了VEGF mRNA表达和促进剂活性.
- 测量VEGF分泌量及其对人静脉内皮细胞 (HUVEC) 管形成的影响.
- 分析了ERK1/2和Akt信号通路的酸化.
主要成果:
- 根据剂量,IGF1在HC11细胞中增加了VEGF mRNA表达,促进剂活性和分泌量.
- 刺激IGF1的条件介质促进了HUVEC管的形成,而HUVEC管是由VEGFR抑制剂阻断的.
- IGF1迅速诱导ERK1/2和Akt酸化,而途径抑制剂减少了VEGF分泌.
结论:
- 通过MEK-ERK1/2和PI3K-Akt信号通路,IGF1可以调节VEGF的产生.
- 这种IGF1-介导的VEGF上调促进乳腺血管生成.
- 这些发现表明,IGF1有助于乳腺发育和哺乳效率的机制.
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