双模式侧流试验用于同时定量抗生素和细菌
Zishan Xu1,2, Ting Wu2, Bingjie Wang1,2
1School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong 510515, China.
Analytical chemistry
|January 31, 2026
概括
这项研究引入了一种新型的双模式侧流检测 (LFA),用于同时检测抗生素 gentamicin 和病原体 Escherichia coli O157. 该试验使用磁SiO2-光探针,以提高复杂样品的灵敏度和可靠性.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 在复杂矩阵中同时检测化学和生物污染物是一个重要的分析障碍.
- 现有的方法往往缺乏全面样本分析所需的灵敏度和多重复合能力.
研究的目的:
- 开发一种双模式侧流测定 (LFA),用于同时超敏感和定量检测抗生素 (gentamicin) 和病原体 (Escherichia coli O157) 污染物.
- 设计一种具有集成丰富和信号放大功能的新型磁性SiO2-光探头 (Fe@Si@QDs).
主要方法:
- 用SiO2中间层制造Fe@Si@QDs探头,以抑制内效应并提高分散性.
- 将三明治和竞争性测试格式集成到一个单一的LFA带上.
- Fe@Si@QDs的抗体功能化,以有效地共捕获目标分析物.
- 通过在不同的测试线上进行光信号调制进行定量检测.
主要成果:
- 达到超敏感检测极限:对大肠杆菌O157的36 cfu/mL和对 gentamicin的2.05 pg/mL.
- 与传统色度LFA相比,显示了500倍以上的灵敏度改善.
- 使用真实环境和食品样本验证了试验的可靠性和稳定性.
结论:
- 开发的双模式LFA平台提供了一个高度灵敏,定量和可靠的解决方案,用于同时检测污染物.
- Fe@Si@QDs 探针设计通过双丰富信号放大有效地提高了测试性能.
- 该平台在现场,家庭和实验室对化学和生物危害的查方面具有广泛的适用性.
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