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相关概念视频

Isothermal Processes01:21

Isothermal Processes

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A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes.
An ideal gas can also undergo isothermal expansion or compression.
For example, consider 1 mole of an ideal gas inside an isolated cylinder at initial volume V...
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Feedback Loops01:01

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In most cases, excessive hormone production is prevented by negative feedback—a loop that starts with a stimulus inducing the release of a particular substance, like a hormone, to maintain a certain level before triggering a signal that results in a decrease in further release of the hormone.
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RACE - Rapid Amplification of cDNA Ends02:35

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Control systems are foundational elements in automation and engineering. They are broadly categorized into open-loop and closed-loop systems. These classifications hinge on the presence or absence of feedback mechanisms, significantly influencing the system's performance, complexity, and application.
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Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis ("steady state"). Examples of these changes include regulation of the level of glucose or calcium in the blood or internal responses to external temperatures. Homeostasis requires  maintaining an internal dynamic equilibrium:
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相关实验视频

Updated: Feb 2, 2026

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
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Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

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颜值测量四组合循环介导的同热放大试验,用于有效检测SARS-CoV-2.

M S Chow1, B P Kee1, P C Lee2

  • 1Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, 50603, Malaysia.

Tropical biomedicine
|January 31, 2026
PubMed
概括

一种新的四组合LAMP测定方法可以快速准确地检测SARS-CoV-2,针对四个基因. 这种方法为大规模和现场查COVID-19提供了有效的替代方案,对于持续的流行病管理至关重要.

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科学领域:

  • 分子生物学分子生物学
  • 病毒学 病毒学
  • 生物技术是生物技术.

背景情况:

  • 由SARS-CoV-2引起的COVID-19大流行,迫使全球采取疫苗接种和社交距离等控制措施.
  • 长期的非药物干预对经济产生了负面影响,新出现的SARS-CoV-2变种挑战了疫苗的有效性.
  • 有效,可扩展的诊断方法对于管理COVID-19和预防大规模爆发至关重要.

研究的目的:

  • 开发一种适合大规模和现场查的SARS-CoV-2高效检测试剂.
  • 创建一个快速诊断工具,以帮助持续管理COVID-19大流行.

主要方法:

  • 开发一个四组组合环介导同热放大 (LAMP) 试验,针对四个SARS-CoV-2基因 (RdRp,S,E,N) 和一个内部控制 (RNaseP).
  • 使用370个来自鼻和/或口口腔的RNA样本验证LAMP测定.
  • 与黄金标准定量逆转录聚合酶连锁反应 (qRT-PCR) 试验的性能比较.

主要成果:

  • 组合LAMP试验的表现与qRT-PCR相提并论.
  • 在Ct值<=35的样品中,获得了98.13%的高灵敏度和100%的特异性.
  • 与qRT-PCR相比,提供了相当短的25分钟的周转时间.

结论:

  • 开发的四组合LAMP试验是一种高度敏感和特定的SARS-CoV-2检测方法.
  • 它的快速周转时间和适合大规模查使其成为COVID-19管理的宝贵工具.
  • 这种测试为及时诊断和控制SARS-CoV-2传播提供了有效的替代方案.