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一个快速反应的NIR光探针用于成像Cys/Hcy在ferroptosis和炎症模型中的成像

Jie Gong¹, Xu-Rong Liu¹, Xuan Liu¹

¹College of Chemistry, Chemical Engineering and Material Science, Soochow University, No. 199 Ren'Ai Road, Suzhou 215123, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|January 31, 2026

概括

研究人员开发了新的近红外探测器,以可视化氨酸和同氨酸的动态. 探测器1a有效地监测线粒体中的这些生物醇,帮助铁和炎症研究.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
化学生物学化学生物学

背景情况:

铁亡是依赖于铁和脂质过氧化的受调细胞死亡.
半氨酸 (Cys) 和同氨酸 (Hcy) 对于铁灭中的氧化还原稳定至关重要.
想象生物乙醇动态对于理解铁和相关的细胞过程至关重要.

研究的目的:

开发新的近红外 (NIR) 光探测器,以可视化氨酸和同氨酸的动态.
为了评估探测器的响应能力,灵敏度,选择性和细胞应用.
在铁亡和炎症期间监测Cys/Hcy水平.

主要方法:

三个基于四基的NIR探针 (1a-1c) 的合成.
探测器属性的表征,包括响应能力,灵敏度,选择性和光增强.
探头1a的应用,用于实时监测活细胞中的线粒体Cys/Hcy.
使用探头1a研究埃拉斯诱导的铁和抑制剂 (Fer-1,NAC) 的作用.
在炎症模型中跟踪生物醇消耗.

主要成果:

探针1a-1c显示出快速响应,高灵敏度和对Cys/Hcy的选择性,具有增强的光和大的Stokes转移.
探测器1a表现出优异的线粒体向能力,低细胞毒性,并实现实时Cys/Hcy监测.
探测器1a可视化了Erastin诱导的铁亡过程中的Cys/Hcy耗尽以及Fer-1和NAC的不同作用.
探头1a在炎症模型中跟踪了线粒体生物醇消耗.

结论:

开发的NIR探头,特别是1a,是实时监测活细胞中Cys/Hcy动态的有效工具.
探测器1a为研究铁亡机制和抑制剂的治疗作用提供了视觉证据.
探测器在追踪炎症中氧化还原失衡的实用性突出了其在相关疾病研究中的潜力.

关键词:

生物乙醇是一种生物乙醇.铁化是铁化的一种.大斯托克斯转移转移.线粒体向的目标是线粒体.接近红外的近红外.

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