来自FilmArray® GI面板的阳性诺罗病毒结果的实际管理
Noémie Rybak1, Philippe Bidet2, Yasmine Benhadid-Brahmi2
1Microbiology Laboratory, Robert-Debré University Hospital, AP-HP, Paris, France.
概括
在儿童中,BioFire® FilmArray®胃肠道面板 (BF-GIP) 的假阳性诺罗病毒结果很常见. 使用周期值和化曲线的算法可以提高诊断准确度,减少不必要的测试.
科学领域:
- 临床诊断 临床诊断 临床诊断
- 分子生物学分子生物学
- 传染病流行病学 传染病流行病学
背景情况:
- 在2024年2月,BioFire® FilmArray®胃肠道面板 (BF-GIP) 用户被提醒可能出现假阳性诺罗病毒结果.
- 建议对 BF-GIP 诺罗病毒阳性结果进行确认测试.
研究的目的:
- 为了评估BF-GIP诺罗病毒结果在儿科患者中的准确性.
- 开发一个决策算法来管理潜在的假阳性结果.
主要方法:
- 用GeneXpert® Norovirus测定方法重新测试了BF-GIP诺基病毒阳性样本.
- 进行了BF-GIP周期值 (Ct) 和化曲线的分析.
主要成果:
- 在127个重新测试的样本中,31.5%是不一致的.
- 非典型的曲线和高Ct值 (>25) 与不一致性相关.
- Ct值≤20强烈预测的一致性.
结论:
- 从BF-GIP获得虚假阳性诺罗病毒结果是常见的.
- 集成Ct值和化曲线分析的算法可以减少不必要的重新测试.
- 这种方法支持准确的诊断,并简化了儿科环境中的实验室工作流程.
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