咖啡酸和大米白蛋白之间的共价相互作用对蛋白质结构和功能的影响
Menglin Guo1, Ran Zhang1, Chenge Zhang1
1College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China.
Food chemistry
|January 31, 2026
概括
咖啡酸 (CA) 与大米白蛋白 (RBA) 结合,增强了其抗氧化和乳化特性. 这种修改改善了用于食品应用的米利用率和蛋白质功能.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 蛋白质化学 蛋白质化学
背景情况:
- 米是一种有价值的副产品,其蛋白质含量未得到充分利用.
- 植物蛋白,如大米白蛋白 (RBA),可以从修改中受益,以提高其功能性质.
- 酸,如咖啡酸 (CA),以其抗氧化和生物活性特性而闻名.
研究的目的:
- 为了研究大米白蛋白 (RBA) 与咖啡酸 (CA) 的共价复合.
- 评估CA修改对RBA结构性,界面性和功能性质的影响.
- 评估RBA-CA复合物的抗氧化活性和体外可消化性,以提高米的利用率.
主要方法:
- 在性溶液中对RBA和CA的共价复合.
- 使用SDS-PAGE,SEM,FTIR和ANS光分析蛋白质结构和交叉链接.
- 评估接口特性,包括发泡性和乳化.
- 通过ABTS和DPPH激素清除试验评估抗氧化活性.
- 模拟体外消化以确定水解速率.
主要成果:
- SDS-PAGE证实了白蛋白分子的交叉链接.
- CA的修改改变了RBA的二次结构和表面的疏水性.
- RBA-CA复合体显示显著增强抗氧化活性 (ABTS增加48.52%,DPPH增加33.08%).
- 通过中低度的RBA-CA复合物改善了RBA的泡性和乳化.
- 试验室消化显示,在CA添加后RBA水解增加.
结论:
- 对CA与RBA的共价键有效地增强了其功能性质.
- RBA-CA复合体显示出改善的抗氧化能力和乳化/发泡能力.
- 这种修改策略为增加在食品中使用大米蛋白增加提供了一个有希望的方法.
- 通过酸结合增强的蛋白质功能支持农业副产品的增值应用.
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