对复杂食物矩阵中益生菌计数的新见解:流细胞计量和盘子计数相关性相关性
Gonzalo Saiz-Gonzalo1, Orlaith O'Connell1, Shima Joy1
1AnaBio Technologies, Carrigtwohill, Co. Cork, Ireland.
Journal of AOAC International
|January 31, 2026
概括
流细胞计提供当天的益生菌计数,列出所有可活细胞以准确标记产品. 这种方法补充了传统的盘子计数,确保食品的精确量化.
科学领域:
- 微生物学 微生物学
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
背景情况:
- 准确的益生菌计数对于确定向宿主输送的剂量至关重要.
- 血小板计数 (PC) 仅检测可培养细胞,可能会低估可活性的细胞,特别是如果受伤或休眠.
研究的目的:
- 验证流细胞计试验,以量化益生菌的活性光单位 (AFU).
- 为了比较AFU计数与传统的盘子计数 (PC) 在零食产品中微封装的Lacticaseibacillus rhamnosus GG.
主要方法:
- 基于AOAC/ICH/USP原则的板数和流细胞计方法的评估.
- 对商业零食产品的精度,准确性,坚固性,特异性和强度的评估.
主要成果:
- 这两种方法都达到了性能目标 (%RSD ≤ 15;AFU恢复100-104%;活/死R2 ≥0.95).
- 流细胞计提供了同一天的结果,高峰恢复 (100-104%) 和线性活/死跟踪 (R2 ≥0.95).
- AFU和殖民地形成单位 (CFU) 计数在±0.5 log以内相当,批量平均差异最小 (≤0.2 log) 并且没有系统偏差.
结论:
- 经过验证的流式细胞计量方法提供了快速的,当天清单的膜完好无损的益生菌细胞.
- 这种方法补充了PC,通过量化所有可活细胞,帮助标签准确性和食品剂量估计.
- 结果支持使用AFU与CFU一起用于零食矩阵中的全面益生菌量化.
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