氧化醇诱导的皮肤结合体完整性的氧化破坏
Alessandra Pecorelli1, Anna Guiotto2, Alice Casoni3
1Department of Environmental and Prevention Sciences, University of Ferrara, Ferrara, Italy; Plants for Human Health Institute, Department of Food, Bioprocessing and Nutrition Sciences, NC State University, Kannapolis, North Carolina, USA.
Free radical biology & medicine
|January 31, 2026
概括
环境污染物会导致氧化应激,损害皮肤屏障. 氧化醇,胆固醇氧化产物,破坏皮肤细胞结合和线粒体功能,损害皮肤的完整性.
科学领域:
- 皮肤病学 皮肤病学
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 皮肤屏障不断暴露于环境压力因素,如空气中的污染物,导致氧化应激.
- 角层,富含胆固醇,特别容易受到氧化损伤.
- 氧化醇是胆固醇氧化的产物,具有促氧化和促炎性质,可以来自环境或饮食来源.
研究的目的:
- 调查有毒氧化醇 (7β-氧化胆固醇和7-胆固醇) 对表皮屏障完整性的影响.
- 为了确定氧醇是否破坏皮肤细胞结合所涉及的关键蛋白质.
- 探索线粒体功能障碍和活性氧物种 (ROS) 在氧化醇诱导的皮肤屏障损伤中的作用.
主要方法:
- 人类角质细胞被用超生理学度的7-基托胆固醇 (7KC) 和7β-氧胆固醇 (7βOHC) 治疗.
- 免疫光分析被用来评估紧结蛋白 (Claudin-1,Zonulin-1) 和粘附结蛋白 (E-cadherin) 的水平.
- 测量了线粒体膜潜力和ROS产量;使用线粒体清理器mitoTEMPO测试了效果.
- 实验使用3D人体皮肤模型进行验证.
主要成果:
- 氧化醇治疗显著降低了角质细胞中Claudin-1,Zonulin-1和E-cadherin的水平.
- 氧化醇暴露降低了线粒体膜潜力,并增加了线粒体ROS产量.
- 线粒体损伤和皮肤结节的破坏,这表明氧的亲氧化活性调解这些效应.
- 在3D皮肤模型中证实了质细胞培养中的发现.
结论:
- 有毒的氧醇通过破坏粘附和紧密的结合,显著地损害了皮肤屏障的完整性.
- 氧化醇诱导的皮肤屏障功能障碍是由增加的氧化应激和线粒体损伤介导的.
- 这些发现强调了氧化是环境压力下皮肤屏障受损的关键因素.
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