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评估三种不同的芝麻ELISA方法来检测和量化食品中芝麻的含量

Chung Y Cho¹, Rakhi Panda¹, Prasad Rallabhandi¹

¹Human Foods Program, Office of Laboratory Operations & Applied Science (OLOAS), Food and Drug Administration, 5001 Campus Drive, College Park, MD 20740, USA.

Journal of food protection

|January 31, 2026

概括

用于检测芝麻的商业ELISA套件显示了可变的性能. 莫里纳加和R-Biopharm套件提供中等精度,而Neogen则提供中等精度.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
检测过敏原的检测方法

背景情况:

芝麻现在是第九个主要的食物过敏原在美国根据2021年FASTER法案规范.
美国食品和药物管理局 (FDA) 使用酶相关免疫吸收分析 (ELISA) 方法来检测食物过敏原.
准确的检测方法对于控制芝麻过敏和确保食品安全至关重要.

研究的目的:

评估商用芝麻ELISA套件的性能,用于检测各种食品矩阵中的芝麻.
评估这些套件是否适用于常规的食物过敏原测试.
为了比较不同ELISA平台的精度,准确性和稳定性.

主要方法:

三个商业的芝麻ELISA套件 (MIoBS,R-Biopharm RIDASCREEN,Neogen Veratox) 进行了测试.
模范食品 (香料混合,胡姆斯,烤面饼干) 添加了芝麻.
分析是由三个独立分析师进行的,仓储后重新测试米以评估强度.

主要成果:

在整个矩阵中,MIoBS和RIDASCREEN套件显示出良好的中间精度.
尼奥根Veratox套件未能检测到胡麻豆在胡麻豆中,并且在其他矩阵中显示出高可变性.
恢复率在套装和食品类型之间有很大的差异,RIDASCREEN高估,MIoBS显示香料混合物的恢复减少.

结论:

商用芝麻ELISA套件的性能因套件,食物矩阵和过敏原度而异.
需要进一步验证,以确保可靠地检测各种食品中的芝麻.
选择ELISA套件对于精确的过敏原量化和监管合规性至关重要.

关键词:

艾丽莎·伊丽莎·伊丽莎评价评价评价食物过敏原食物过敏原芝麻:芝麻是一种

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