在禾中找到针:测量循环瘤DNA (ctDNA) 以指导瘤学治疗决策
Genia Dubrovsky1, Noah Earland2, Peter Harris3
1Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States; Pittsburgh VA Medical Center, Pittsburgh, PA, United States.
Methods in cell biology
|January 31, 2026
概括
液体活检是一种非侵入性癌症检测方法,使用循环瘤DNA (ctDNA) 显示出有前途. 本综述涵盖了ctDNA测定程序和癌症护理中的应用,从查到治疗指南.
科学领域:
- 在瘤学瘤学.
- 分子诊断学 分子诊断
- 生物化学 生物化学
背景情况:
- 液体活检是一种使用生物流体检测癌症的新兴非侵入性技术.
- 它包括分析循环中的核酸,蛋白质和细胞的各种测试.
- 循环瘤DNA (ctDNA) 正因其在癌症管理方面的潜力而受到显著关注.
研究的目的:
- 审查循环瘤DNA (ctDNA) 测试的程序性方面.
- 探索ctDNA测定在癌症治疗中的多样化应用.
- 介绍ctDNA分离,检测和量化的协议.
主要方法:
- 关于液体活检和ctDNA测定现有文献的审查.
- 讨论生物流体的实用性,技术考虑和测试格式.
- 介绍了包括向ctDNA qPCR在内的协议.
主要成果:
- ctDNA测定为癌症查和监测提供了一种非侵入性的方法.
- 这些测试有助于评估治疗反应和治疗决策.
- 各种生物流体和测试格式适用于ctDNA分析.
结论:
- 使用ctDNA的液体活检是一个快速发展的领域,在瘤学中具有广泛的应用.
- 了解程序方面和技术考虑对于测试实施至关重要.
- 包括qPCR等方法在内的ctDNA分析对于个性化癌症护理至关重要.
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