来自TE的TSS的架构和进化特征在人类基因组中塑造了组织特异性促进剂活性
Ya Zhang1, Yahan Fan2, Huang Wu2
1Shanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China. zhangaya1225@tongji.edu.cn.
Nature communications
|January 31, 2026
概括
人类基因组中的可移植元素作为替代促进体,驱动组织特异性基因表达. 年轻的元素表现出更强烈,更集中的转录,而老的元素则更广泛,更不活跃.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 可转移元素 (TE) 在人类基因组中非常丰富.
- 试验剂越来越多地被认为是替代性促进剂的来源.
- 人体组织中TE转录活性和调节潜力的全部程度尚不清楚.
研究的目的:
- 构建一个全面的转录起点地图 (TSSs) 来自人体组织中TEs.
- 调查 TE 衍生 TSS 的监管潜力的特征.
- 了解TE促进者活动的进化动态.
主要方法:
- 整合了来自115个人类生物样本的高分辨率RAMPAGE数据.
- 构建一个包含26,056个TE衍生TSS的综合图谱.
- 对 TE 子家族的遗传学分析,以评估促进体强度和转录精度.
主要成果:
- 鉴定了26,056种TE衍生的TSS,有助于组织特异性基因表达.
- 这些TSS中很大一部分来自于灵长类动物和人类类动物特有的元素.
- 来自TE的TSS表现为聚焦架构,TATA盒子丰富和CpG岛屿耗尽.
- 遗传学分析揭示了TE子家族中促进体强度和精度的梯度.
- 进化上较年轻的TE具有针对性强的转录的内在动机;较老的TE显示更广泛的启动和较低的活动.
结论:
- 转基因是人类基因组中替代性促进体使用和组织特异性基因表达的重要贡献者.
- 转基因中促进体特征的演化和保存影响了它们作为功能促进体的表现.
- 在灵长类动物中,特E,特别是年轻的特E,在血统特定的监管创新中发挥着作用.
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