一个高参数的光谱细胞检测协议,用于免疫子集和功能特征的冷保存的人类血液与49个生物标志物
Austin P Wright1, Marissa Lindaman2, Elaine Huang2
1Department of Cell, Development & Cancer Biology, Oregon Health & Science University and Knight Cancer Institute, Portland, OR 97239, USA; Knight Cancer Institute, Portland, OR 97201, USA.
STAR protocols
|February 1, 2026
概括
这项研究详细介绍了一种49色的光谱流细胞计方案,用于对人类血液进行全面的免疫分析. 它可以在冷保存样本中详细分析白细胞系,激活和免疫检查点,用于临床试验研究.
科学领域:
- 免疫学 免疫学 免疫学
- 临床试验 临床试验
- 生物技术是生物技术.
背景情况:
- 在临床试验中评估治疗干预措施时,全面的免疫分析至关重要.
- 评估白细胞系,激活状态和免疫检查点表达提供了对免疫反应的关键见解.
研究的目的:
- 介绍使用光谱流细胞测量方法对人类血液进行高维免疫分析的详细方案.
- 为了能够对冷保存的布菲外套样本进行可靠的分析,以便进行深度免疫类型定型.
主要方法:
- 使用了49色的光谱流细胞计抗体面板.
- 开发了一种解,洗和染色冷保存的循环免疫细胞的协议.
- 提供了关于数据采集用于高维分析的指导.
主要成果:
- 建立了一种可复制的方法,用于全面的免疫细胞分析.
- 证明了该协议适用于评估白细胞子集和免疫检查点表达的适用性.
- 从冷保存的布菲外套样本中实现了详细的免疫型定型.
结论:
- 提出的协议为临床研究中的深度免疫类型定型提供了一个强大的工具.
- 这种方法对研究癌症免疫学和其他与免疫相关的征兆有价值.
- 在治疗干预研究中促进标准化免疫反应评估.
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