在芯片上的口腔失生性病变的初步模型
Roberto Plebani1, Tania Vanessa Pierfelice1, Emira D'Amico1
1Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio", Via dei Vestini, Chieti 31,66013, Italy.
Tissue & cell
|February 1, 2026
概括
这项研究提出了一种新的器官芯片模型,用于研究口腔发育不良. 该模型成功模仿了关键的细胞相互作用,为癌前口腔病变研究提供了一个新的工具.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
背景情况:
- 目前的口腔发育不良体外模型缺乏生理上相关的组织接口和微环境线索.
- 开发先进的模型对于了解癌前口腔病变及其进展至关重要.
研究的目的:
- 开发和验证癌前口腔病变 (OD-OoC) 的初步器官芯片 (OoC) 模型.
- 在微流体装置内创建一个3D体外模型,复制关键细胞相互作用和微环境因素.
主要方法:
- 使用双通道微流体装置,在一个原I涂层膜上共同培养人类脉内皮细胞 (ECs),人类牙纤维细胞 (hGFs) 和异形口腔角质细胞 (DOKs).
- 细胞相互作用和迁移在五天内被确定,随后对特定细胞标记物 (波多普拉宁,Trop2,VE-cadherin) 进行免疫光染色和共聚焦显微镜分析.
- 通过评估E-cadherin,EpCAM和Trop-2表达,进行了DOKs的表型表征.
主要成果:
- OD-OoC模型成功地确定了EC,hGF和DOK之间的相互作用,观察到纤维细胞向内皮层迁移.
- 失塑性口腔角质细胞表现出明显的表型标记,包括Trop-2阳性和可变的E-cadherin/EpCAM表达,使它们与健康的上皮细胞有所区别.
- 该模型证明了监测表皮细胞表型变化和细胞穿过膜迁移的能力.
结论:
- 与传统的体外试验方法相比,开发的OD-OoC模型为研究口腔发育不良提供了更具生理相关性的平台.
- 这种模型在体外监测癌前口腔病变和推进口腔癌研究方面具有重大潜力.
- 进一步的应用可能包括口腔瘤学中的药物查和个性化医疗方法.
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