低温无酸盐肉的多酶级联染色:通过NOS-YkuN-YumC和葡萄糖脱酶的催化
Qing Xiao1, Yali Zhou1, Huiting Luo1
1Joint Research Center for Food Derived Functional Factors and Synthetic Biology of IHM, Hefei University of Technology, Hefei 230601, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Meat science
|February 1, 2026
概括
使用葡萄糖脱酶 (GDH) 和NOS-YkuN-YumC的新型多酶系统在10°C时有效地产生肉色. 这种无酸盐的方法增强了尼特肌球蛋白的形成,并稳定了肉制品的颜色.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 酶学 是一种酶学.
背景情况:
- 传统的肉固依赖于亚酸盐,这些亚酸盐与健康问题有关.
- 在没有酸盐的肉制品中开发稳定,有吸引力的颜色,特别是在冷藏下,仍然是一个挑战.
- 酶级联系统为食品系统中的有针对性的生物化学转换提供了潜在的替代方案.
研究的目的:
- 研究多酶级联系统的有效性,包括葡萄糖脱酶 (GDH) 和融合酶 (NOS-YkuN-YumC),用于在低温 (10°C) 下培养肉色.
- 评估该系统在肉制品中促进酸肌球蛋白 (NO-Mb) 形成和颜色稳定性的能力.
- 为了展示一种无酸盐的方法来增强肉的颜色.
主要方法:
- 复合型葡萄糖脱酶 (GDH) 得到了Bacillus subtilis 168.8的表达和净化.
- 一种融合酶,NOS-YkuN-YumC,与GDH一起在10°C化的碎肉系统中使用.
- 通过测量a*值来评估颜色的发育,并使用UV-Vis光谱学确认了尼特胺蛋白的形成.
- 与对照组 (仅使用NOS-YkuN-YumC) 和酸盐治疗组进行了比较分析.
主要成果:
- 纯化GDH在尼古丁胺胺氨基二核酸盐 (NADPH) 再生方面表现出高效率.
- 与单独NOS-YkuN-YumC治疗相比,补充GDH显著增加了碎肉的a*值.
- 添加GDH有效地促进了尼特肌球蛋白的形成,并保持了在熟肉中较高的a*值,表明颜色稳定性得到了增强.
- 酶系统显示了与酸盐处理相似的颜色发育,没有显著差异.
结论:
- 多酶级联系统,特别是添加了GDH,在低温下成功增强了肉的颜色发育.
- GDH促进NADPH的产生,促进NOS-YkuN-YumC的催化活性,以改善NO生成和尼特肌球蛋白的形成.
- 这项研究提出了一种有希望的无酸盐酶方法,用于在冷藏下实现肉制品的理想和稳定的颜色.
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