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转基因植物中抗人类PD-1单克隆抗体的表达和功能分析

Chae Eun Lee¹, Sohee Lim¹, Da Won Lee¹

¹Department of Urology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.

Journal of biotechnology

|February 1, 2026

概括

转基因烟草植物产生了pembrolizumab,这是一个功能性抗PD-1抗体. 这种植物性抗体针对免疫细胞PD-1和瘤细胞iPD-1,抑制癌细胞的增殖.

科学领域:

生物技术是生物技术.
免疫学免疫学免疫学
植物科学植物科学

背景情况:

基于植物的生物制药生产为治疗性抗体制造提供了具有成本效益和可扩展的方法.
布罗利祖马布是一种单克隆抗体 (mAb),向编程细胞死亡蛋白-1 (PD-1),在癌症免疫治疗中至关重要.
一种在瘤细胞中表达的新型内在PD-1 (iPD-1) 变异呈现出一种新的治疗标.

研究的目的:

开发一种植物性表达系统,以产生一种功能性的抗PD-1 mAb.
评估植物衍生抗PD-1 mAb (mAb^P PD-1) 对经典PD-1和iPD-1的疗效.
研究mAb^P PD-1在癌细胞中的抗增殖机制.

主要方法:

转基因Nicotiana tabacum (烟草) 植物的生成以表达pembrolizumab.
使用SDS-PAGE和免疫洗,净化和验证植物衍生抗PD-1 mAb (mAb^P PD-1).
功能测定包括ELISA,免疫组织化学,基于细胞的增殖测定和西部斑分析.

主要成果:

成功净化并验证了植物衍生抗PD-1 mAb (mAb^PPD-1),具有强烈的与人类PD-1的结合亲和力.
mAb^P PD-1 在膀癌细胞上有效结合了iPD-1并抑制了它们的增殖.
作用机制包括抑制ERK酸化,表明MAPK信号通路的调制.

结论:

转基因烟草植物为具有成本效益和可扩展的功能性抗PD-1抗体生产提供了一个可行的平台.
来自植物的抗PD-1抗体具有强大的免疫调节和抗增殖特性.
PD-1和iPD-1的双重向突出了这些植物性抗体在癌症免疫治疗中的治疗潜力.

关键词:

反PD-1 抗体的抗体.免疫检查点抑制剂免疫检查点抑制剂佩姆布罗利兹马巴姆 (Pembrolizumab) 是一种制品.植物性表达系统植物性表达系统再组合蛋白质是一种重组蛋白质.

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