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Updated: Feb 3, 2026

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Published on: July 15, 2018

从常规实践中新鲜冷的癌样本中RNA完整性的危险因素 (UroCCR 9)

G Margue¹, G Capon², B C Niort³

¹Department of Urology - CHU Bordeaux, Bordeaux, France; I.CaRe Bordeaux - BRIC INSERM U1312, Bordeaux, France; Kidney Cancer group of the Oncology Committee of the French Association of Urology, Paris, France.

The French journal of urology

|February 1, 2026

概括

从新鲜冷的细胞癌 (RCC) 组织中可以获得用于转录组分析的高质量的RNA. 瘤生物学,而不是程序延迟,主要影响常规生物银行中的RNA完整性.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
这是生物银行.

背景情况:

高质量的生物样本对于细胞癌 (RCC) 的转录组分析至关重要.
在常规RCC手术环境中影响RNA完整性的现实世界因素尚未得到充分证实.
这项研究评估了冷保存的RCC组织中的RNA数量和质量,并确定了降解因子.

研究的目的:

评估冷保存细胞癌 (RCC) 组织中的RNA数量和质量.
在常规手术环境中识别影响RNA降解的因素.
在RCC中为转录基因研究提供生物银行策略的信息.

主要方法:

来自UroCCR网络 (2005-2012) 的160名清细胞RCC患者的回顾性分析.
预期收集和快速结瘤和匹配的正常脏组织.
自动RNA提取,纳米滴光谱测定度,以及RNA质量评分 (RQS) 用于质量评估.
使用随机拦截的多变量线性回归模型.

主要成果:

从310个样本中的302个成功提取RNA (97.4%).
93%的样本的RNA度超过25 ng/μL; 78.4%的样本符合定量和质量标准 (RQS ≥6).
瘤组织的RNA质量明显高 (RQS平均值8.33),而不是匹配的正常组织 (RQS平均值6.66).
术前栓塞独立地与较低的RNA质量相关 (p < 0.001).
冷延迟 (平均30分钟) 和储存时间与RNA降解无关.

结论:

在标准的临床条件下,可以从新鲜冷的RCC组织中可靠地提取高质量的RNA.
瘤生物学是RNA完整性的一个更重要的决定因素,而不是短时间的手术延迟.
这些发现支持开发用于RCC研究的生物知情生物银行策略.

关键词:

质量的RNA质量RNA质量细胞癌是细胞癌.生物银行是生物银行.化保存的方法

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