开发LMW基多稳定黄金纳米粒子DNA生物传感器功能化与Vibrio parahaemolyticus检测的Thiolated探头
Jessa D Alonzo1, Kristopher Ray S Pamintuan2, Khyle Glainmer N Quiton2
1School of Graduate Studies, Mapúa University, Muralla Street, Intramuros, Manila 1002, Philippines; School of Chemical, Biological, and Materials Engineering and Sciences, Mapúa University, Muralla Street, Intramuros, Manila 1002, Philippines; Department of Chemical Engineering, College of Engineering, Mariano Marcos State University, City of Batac, 2906, Ilocos Norte, Philippines.
这项研究介绍了一种新型的金纳米粒子生物传感器,用于检测中的急性肝胰腺性缩疾病 (AHPND). 这种快速,设备精简的诊断工具有效地识别了Vibrio parahaemolyticus,为水产养殖疾病监测提供了潜力.
科学领域:
- 纳米技术纳米技术
- 生物技术是生物技术.
- 水生动物卫生 水生动物卫生
背景情况:
- 急性肝胰腺坏死病 (AHPND) 对全球养殖业构成重大威胁.
- 携带pirA/pirB基因的Vibrio parahaemolyticus菌株是AHPND的主要致病原体.
- 当前的诊断方法可能耗时,需要专门的设备,限制了它们在现场环境中的使用.
研究的目的:
- 开发和验证基于金纳米粒子 (AuNPs) 的色度生物传感器,用于快速检测Vibrio parahaemolyticus中的pirA基因.
- 将特定序列DNA杂交与聚合物介导的合体稳定进行整合,以提高生物传感器性能.
- 在资源有限的水产养殖环境中为AHPND监测建立一个强大的,设备精简的诊断平台.
主要方法:
- 酸盐减少的黄金纳米粒子 (AuNPs) 用化ssDNA探针进行了功能化,并用低分子量酸盐稳定.
- 使用循环介导同热放大 (LAMP) 来放大目标 pirA 基因.
- 检测涉及通过视觉颜色变化和UV-Vis光谱学评估AuNP-Probe-Chit结合物的盐诱导聚合.
主要成果:
- 优化的AuNP-Probe-Chit生物传感器在与LAMP增强DNA杂交后,在阳性和阴性样本之间展示了清晰的视觉和光谱区分.
- 生物传感器对 pirA 基因表现出高特异性,没有与其他 Vibrio 物种交叉反应.
- 灵敏度达到与LAMP放大值相当的水平,可靠地检测到≥10−1 ng的目标DNA.
结论:
- 一个新的,基于设备薄金纳米粒子的色度生物传感器已成功开发用于AHPND检测.
- 整合系统的化ssDNA探针,基托稳定,和AuNPs提供了一个快速和可见的检测方法.
- 这种生物传感器在水产养殖中具有有效的AHPND监测的巨大潜力,特别是在资源有限的环境中.
更多相关视频
09:34Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
08:38Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells
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