使用光共聚焦显微镜对希尔施普朗格病的诊断评估:可行性研究
Yannick Braun1, Elise Gradhand2, Florian Friedmacher1
1Goethe University Frankfurt, University Hospital, Department of Pediatric Surgery and Pediatric Urology, Frankfurt, Germany.
Journal of pediatric surgery
|February 1, 2026
概括
光共聚焦显微镜 (FCM) 提供了一种快速的,手术内诊断的方法,通过成像未固定肠道组织来诊断赫什普朗格病 (HD). 它的准确性与冷部分相当,有助于为儿科患者进行手术规划.
科学领域:
- 儿科手术 儿科手术
- 胃肠病学 胃肠病学
- 医疗成像医学成像
背景情况:
- 赫施普隆病 (Hirschsprung disease,简称HD) 是一种先天性疾病,由于缺少神经,导致肠道阻塞.
- 准确确定角质细分长度对于手术成功至关重要.
- 组织学检查是目前评估结结瘤程度的标准.
研究的目的:
- 评估光共聚焦显微镜 (FCM) 的可行性和诊断准确性,用于在手术期间评估赫施普隆病.
- 将FCM的诊断性能与传统组织学方法进行比较.
- 探索FCM作为一种快速,非破坏性HD成像技术.
主要方法:
- 对接受HD手术的儿科患者进行前性分析.
- 使用FCM进行非固定组织样本的手术内成像.
- 将FCM发现与冷截面和甲固定嵌体 (FFPE) 组织学进行比较.
- 图像质量和plexuses可评估性的评估.
主要成果:
- 在所有样本中,FCM与FFPE组织学的诊断一致性为78.5%,在可评估样本中为80.9%.
- 冷部分的同意率更高 (86.9%总体,86.4%可评估),但与FCM相比没有统计学意义 (p=0.49).
- 在不改变组织完整性的情况下,FCM提供了快速的手术内成像.
结论:
- FCM是一种可行且易于实施的方法,用于在HD中进行手术期间识别正常肠道和角质肠道.
- FCM的诊断准确性与可评估样本中的冷部分相当.
- 在对专家病理学家的访问有限的环境中,FCM提供了优势,可保存组织以供未来分析.
相关概念视频
Confocal Fluorescence Microscopy
21.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.1K
Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies
3.2K
Assessing and diagnosing Chronic Obstructive Pulmonary Disease (COPD) involves a detailed approach that includes a comprehensive review of medical history, physical examination, and a variety of diagnostic tests. This thorough evaluation is essential to ensure an accurate diagnosis and guide effective management strategies.
Medical History
Medical History
3.2K
Rheumatic Heart Disease II: Clinical Manifestations and Diagnostic Studies
587
The key clinical manifestations of Rheumatic heart disease (RHD) include several distinct cardiac symptoms.Carditis, a hallmark of acute rheumatic fever, involves inflammation of the heart's endocardium, myocardium, and pericardium. Chronic RHD often results from recurrent episodes of carditis. Its symptoms include the following:Murmurs are caused by valvular damage, especially to the mitral and aortic valves. Mitral stenosis or regurgitation is common, with characteristic heart murmurs...
587
Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
596
Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
596
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
742
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
742
Appendicitis-II: Diagnostic Studies and Management
570
Diagnosing and managing appendicitis requires a structured and comprehensive approach that spans from initial assessment to postoperative care. Here is an overview of the process:
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
570


