转录基因分析确定VvMYB90和VvNAC92是三种葡萄品种中介于安托西亚尼生物合成和果色差异化的关键调节者
Han Lin1, Kaili Mao1, Shilong Chai1
1College of Horticulture / Institute of Storage, Transportation and Preservation of Horticultural Products, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
概括
这项研究通过分析三种品种的安托西亚宁合成来研究葡萄的颜色差异化. 参与黄类生物合成的关键基因在水果发育过程中被上调,揭示了调节网络.
科学领域:
- 植物生物学 植物生物学
- 基因组学就是基因组学.
- 生物化学 生物化学
背景情况:
- 葡萄的颜色差异化对果实质量至关重要.
- 调节葡萄中安东氨酸合成和颜色发育的机制尚未完全理解.
研究的目的:
- 阐明三种不同的葡萄品种 ("Shine Muscat"",Gaoqiansui"",Moldova") 中的安托西亚尼合成和颜色分化的分子机制.
主要方法:
- 在三个葡萄品种的不同发育阶段进行转录基因分析.
- 重量基因同表达网络分析 (WGCNA) 和相关性网络构建.
- 识别转录因子 (TFs) 和它们的向结构基因在氨酸生物合成途径.
主要成果:
- 差异表达基因 (DEGs) 在烯胺和黄胺生物合成途径中显著丰富.
- 在和成熟期间观察到的与途径相关基因的升级,特别是在"摩尔多瓦" (ME) 品种中.
- 构建了一个TF结构基因调节网络,确定VvMYB90和VvNAC92作为氨酸合成基因 (例如,UFGT,CHS) 的潜在调节者.
结论:
- 这项研究揭示了安东氨酸生物合成和葡萄果颜色差异化的分子基础.
- 确定了关键的转录因子及其向基因,为调节葡萄皮颜色提供了洞察力.
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