一种简单的保存方法,用于环境细菌的短期和中期存储
John E Moore1, Beverley Cherie Millar1
1School of Medicine, Dentistry and Biomedical Sciences, The Wellcome-Wolfson Institute for Experimental Medicine (WWIEM), Queen's University, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK; Laboratory for Disinfection and Pathogen Elimination Studies, Northern Ireland Public Health Laboratory, Belfast City Hospital, Lisburn Road, Belfast BT9 7AD, Northern Ireland, UK.
Journal of microbiological methods
|February 1, 2026
概括
一种新的方法允许在5°C下使用标准盘计数来储存环境细菌六个月. 这种简单的技术确保了未来研究的细菌生存能力.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
背景情况:
- 环境细菌对于生态过程至关重要.
- 长期微生物研究和生物银行需要有效的储存方法.
研究的目的:
- 开发和评估一种简单,具有成本效益的方法,用于存储环境细菌长达六个月.
- 评估各种环境细菌在短期到中期储存后的生存能力.
主要方法:
- 从绿色空间中分离出来的50种环境细菌在标准板计数 (SPC) 上培养.
- 在30°C进行72小时的化,然后堆叠和包装板.
- 在黑暗中在5°C保存六个月.
主要成果:
- 所有环境细菌分离物在储存六个月后仍然具有活力.
- 该方法在新鲜的SPC板上进行亚培养时显示出高回收率.
- 该程序需要最小的实验室空间和资源.
结论:
- 描述的方法为保护环境细菌提供了一种可靠且易于使用的方法.
- 这种技术有助于对环境中孤立物进行常规档案,以便在未来进行研究.
- 储存方法简单,廉价,可适应各种实验室环境.
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