相关实验视频

Updated: Feb 3, 2026

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Published on: August 27, 2013

在冷保存后对红细胞活力的基于表面声波的评估

Xi Chen^1,2, Zhaojiang Chen¹, Shiqing Liu¹

¹College of Physics and Electronic Information Engineering, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.

Analytical chemistry

|February 2, 2026

概括

一个新的表面声波 (SAW) 传感器提供非侵入性,实时评估红细胞 (RBC) 存活能力的冷保存后. 这种无标签的方法实现了高吞吐量和灵敏度,克服了当前技术的局限性.

科学领域:

生物医学工程生物医学工程
传感器技术传感器技术
血液学血液学血液学

背景情况:

红细胞 (RBC) 输血至关重要,需要冷保存以保持供应稳定.
冷保存可能会损害红细胞的完整性,需要准确的解后生存能力评估.
现有的可行性评估方法缺乏非侵入性,高吞吐量,实时能力,无标签运行和抗干扰能力.

研究的目的:

开发和验证一种新的表面声波 (SAW) 传感器系统,用于无标签,实时的RBC可行性评估.
为了证明传感器能够区分活跃的红细胞,受损的细胞和溶液.
为了比较传感器的性能与既定方法,如紫外光谱光度测量.

主要方法:

利用与SAW传感器集成的微流体通道来分析离心后的RBC悬浮物.
监测检测和参考通道之间的差异信号响应 (振幅相).
根据振幅差异反应的比率来定义RBC活力.

主要成果:

SAW 传感器可靠地将包装的红细胞与血红蛋白溶液区分开来,并且对红细胞幽灵不敏感.
红细胞活力测量与紫外线光谱测量有很强的相关性 (R2 = 0.99625).
实现了高评估率 (~5.1 × 105 细胞/秒) 和低检测极限 (0.05 μL),超过了UVS灵敏度.

结论:

SAW 传感器提供了一个无标签,高通量,实时和非破坏性的方法来评估RBC可行性.
这项技术克服了当前方法的局限性,提供了增强的灵敏度和芯片上的分离能力.
开发的系统具有改善血液储存和输血医学的巨大潜力.

更多相关视频

Fabrication of Surface Acoustic Wave Devices on Lithium Niobate

Fabrication of Surface Acoustic Wave Devices on Lithium Niobate

Published on: June 18, 2020

Fabrication of Nanoheight Channels Incorporating Surface Acoustic Wave Actuation via Lithium Niobate for Acoustic Nanofluidics

Fabrication of Nanoheight Channels Incorporating Surface Acoustic Wave Actuation via Lithium Niobate for Acoustic Nanofluidics

Published on: February 5, 2020

相关概念视频

The Wave Nature of Light

The Wave Nature of Light

The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms and was able to demonstrate that white light consists of the individual colors of the rainbow combined together. Newton explained his optics findings in terms of a "corpuscular" view of light, in which light was composed of streams of extremely tiny particles traveling at high speeds according to Newton's laws of motion.

Cell-surface Signaling

Cell-surface Signaling

Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.

Assessing Blood pressure in the Leg

Assessing Blood pressure in the Leg

Proper measurement of leg blood pressure is a critical skill for healthcare providers, ensuring precise and reliable readings. When performed correctly, this procedure informs patient care and enhances the efficacy of interventions. The following text outlines step-by-step guidelines to measure blood pressure in the leg, providing clarity and ease of understanding for practitioners.
Preparation:

Red Algae

Red Algae

Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...

Assessing Blood pressure using a doppler ultrasound

Assessing Blood pressure using a doppler ultrasound

To obtain accurate blood pressure measurements in clinical settings, especially when traditional methods are insufficient, healthcare professionals utilize the Doppler ultrasound technique. This method uses high-frequency sound waves to detect blood flow within the arteries, which is crucial for patients with conditions that complicate circulatory system assessment.
Pre-Procedural Guidelines for Doppler Ultrasound Blood Pressure Assessment:
Preparation of Equipment:

Wave Parameters

Wave Parameters

The simplest mechanical waves are associated with simple harmonic motion and repeat themselves for several cycles. These simple harmonic waves can be modeled using a combination of sine and cosine functions. Consider a simplified surface water wave that moves across the water's surface. Unlike complex ocean waves, in surface water waves, water moves vertically, oscillating up and down, whereas the disturbance of the wave moves horizontally through the medium. If a seagull is floating on the...