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相关概念视频

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Nuclear Stability03:18

Nuclear Stability

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Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
23.3K
RNA Stability01:53

RNA Stability

35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Stability01:28

Stability

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The time response of a linear time-invariant (LTI) system can be divided into transient and steady-state responses. The transient response represents the system's initial reaction to a change in input and diminishes to zero over time. In contrast, the steady-state response is the behavior that persists after the transient effects have faded.
The stability of an LTI system is determined by the roots of its characteristic equation, known as poles. A system is stable if it produces a bounded...
418
Peptide Bonds02:43

Peptide Bonds

83.0K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
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相关实验视频

Updated: Feb 3, 2026

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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林/侧链C-H/O相互作用稳定了cis-林.

Harrison C Oven1, Himal K Ganguly1, Neal J Zondlo1

  • 1Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA. zondlo@udel.edu.

Physical chemistry chemical physics : PCCP
|February 2, 2026
PubMed
概括

C-H/O 相互作用稳定了蛋白质中的 cis-proline 构造,特别是在 Glu-Pro 序列. 这些相互作用对于素和三素残留物也至关重要.

科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 计算化学计算化学

背景情况:

  • cis-proline构成对蛋白质的结构和功能至关重要.
  • 了解稳定cis-proline的因素对于蛋白质工程和药物设计至关重要.

研究的目的:

  • 为了确定稳定 cis-proline 形状的局部蛋白质结构.
  • 研究C-H/O相互作用在稳定cis-proline中的作用.

主要方法:

  • 蛋白质数据库 (PDB) 结构的生物信息学分析.
  • 密度函数理论 (DFT) 的计算.

主要成果:

  • 在cis-proline构成中,在侧链氧和Pro C-Hα之间确定了C-H/O相互作用.
  • 这些相互作用在Glu-Pro序列中最具稳定性,显示出高频率的cis-proline.
  • DFT计算证实了C-H/O相互作用的稳定作用,特别是在Glu和Asp等离子残留物中.
  • C-H/O相互作用还使cis-proline稳定在phosphoserine-proline和phosphothreonine-proline中,其dianonic形式的相互作用更强.

结论:

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  • C-H/O 相互作用是 cis-proline 构造的一个关键稳定因素.
  • 这些发现解释了胺结合异构体在聚烯-氨酸和聚氨酸-氨酸序列中的较高激活屏障.
  • 这些相互作用也可能稳定其他cis胺基键,突出显示它们的功能重要性.