相关实验视频
Updated: Feb 4, 2026

11:05
Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
38.2K
在氧化应激下通过cPLA2和STAT3酸化介导的S1PR3无活化加剧脑血管内皮细胞的透性
1Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, Nanjing, China.
Basic & clinical pharmacology & toxicology
|February 2, 2026
概括
氨酸-1-酸盐受体-3 (S1PR3) 保护大脑血管系统免受氧化应激. 失活S1PR3会使内皮壁功能障碍和细胞损伤恶化,强调其保护作用.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 在脑缺血期间,脑血管完整性至关重要.
- 氨酸-1-酸盐受体-3 (S1PR3) 在保持这种完整性方面发挥着作用.
- 氧化应激显著影响内皮细胞功能和屏障特性.
研究的目的:
- 研究S1PR3在氧化应激诱导的大脑血管内皮细胞透性的作用.
- 阐明涉及S1PR3失活的潜在分子机制.
主要方法:
- 使用了bEnd3细胞,一种小鼠大脑内皮细胞系.
- 使用S1PR3阻塞和基因敲击 (透过lentivirus介导).
- 评估了内皮通透性,ZO-1再分配,活性氧物种 (ROS) 水平,细胞活力和西部斑点用于通路激活 (p38,ERK,cPLA2,JNK,STAT3酸化).
主要成果:
- S1PR3阻塞或淘汰会加剧过氧化 (H2O2) 引起的超透性,ZO-1损失,ROS积累和细胞活力降低.
- 通过S1PR3的失活,增强了p38,ERK,cPLA2,JNK和STAT3.3的H2O2诱导酸化.
- 在氧化应激下,S1PR3通过cPLA2-和STAT3-依赖的通路作为内皮屏障完整性的守护者.
结论:
- 在氧化应激期间,S1PR3对于维持脑血管内皮屏障功能至关重要.
- 通过特定的信号通路,S1PR3的失活会增强氧化应激引起的损伤.
- 针对S1PR3可能为涉及脑血管氧化应激的疾病提供治疗潜力.
相关概念视频
Phosphorylation
53.9K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
53.9K
X-Inactivation
41.8K
The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
41.8K
Responses to Heat and Cold Stress
14.8K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.8K
Oxidation Numbers
42.6K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.6K
Activation and Inactivation of G Proteins
11.5K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
11.5K
Pyruvate Oxidation
168.8K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
168.8K

