评估以番茄为基础的农业工业副产品作为Trichoderma harzianum种植的基质和潜在的生物化剂
Antonia Esposito1, Valeria Scala2, Nikolay Vassilev3
1Research Centre for Agriculture and Environment, Council for Agricultural Research and Economics (CREA-AA), Florence, Italy.
这项研究优化了Trichoderma harzianum (T. harzianum) 使用番茄副产品加斯帕乔生物化剂的生产. 新媒介增强了生物质,子产量和多功能活动,支持成本效益高,可持续的生物肥料开发.
科学领域:
- 农业微生物学 农业微生物学
- 生物技术是生物技术.
- 菌类学 菌类学是指菌类学.
背景情况:
- 特里科德玛哈尔齐安 (Trichoderma harzianum) 是一种关键的生物控制剂,具有作为多功能生物化剂的潜力.
- 工业规模的生产在优化生物质和子产量方面面临着具有成本效益的挑战.
- 利用食品工业的副产品可以降低生产成本,并支持循环经济原则.
研究的目的:
- 评估一种新型媒介,将传统生长媒介与T. harzianum子/菌体生产的gazpacho结合起来.
- 评估加斯帕乔补充剂对T. harzianum生物质和胞的影响.
- 研究在加斯帕乔补充介质上种植的T. harzianum的多功能活动,包括盐耐受性,酸盐溶解,以及对番茄病原体的对抗性.
主要方法:
- 在传统的土壤中种植T. harzianum,并补充不同度的加斯帕乔 (3%,6%,10%).
- 在固体和浸泡状态下发酵,含有或不含有100毫米NaCl.
- 评估生物质和化的产量.
- 通过双培养试验评估盐应激耐受性,岩石酸盐溶解,以及对Botrytis cinerea,Fusarium oxysporum和Pyrenochaeta lycopersici的对抗活性.
主要成果:
- 含有3%和6%加斯帕乔的介质在固体和浸泡发酵中显著增加了T. harzianum生物质和化.
- 通过将3-6%的加斯帕乔与100毫米的NaCl相结合,生物质和胞进一步增强.
- T. harzianum在主要番茄病原体,特别是B. cinerea (78.40%),表现出显著的酸盐溶解和超过70%的菌株生长抑制.
- 这种真菌在固体介质中耐受100mM NaCl.
结论:
- 用6%的加斯帕乔补充传统媒介可以优化T. harzianum生物质和子生产.
- 加斯帕乔补充的介质增强了T. harzianum的多功能生物控制和促进植物生长的活动.
- 这种方法为生产T. harzianum生物剂提供了成本效益高的策略,促进可持续农业和循环经济.
- 在加斯帕乔种植的T. harzianum的盐分耐受性使其适合于具有挑战性的农业环境.
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