对非致病性工业相关细菌的病原性潜力的评估
Katrine Nøhr-Meldgaard1,2, Carsten Struve1, Hanne Ingmer2
1Chr. Hansen A/S, Hørsholm, Denmark. Now Novonesis A/S, Lyngby, Denmark.
Access microbiology
|February 2, 2026
概括
评估工业细菌的病原性潜力是一项挑战. 基因组查和表型分析成功地将真正的毒性因子与非致病菌株的利基因子区分开来.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 基因组学就是基因组学.
背景情况:
- 使用针对已知的病原体设计的方法,很难评估非病原性细菌的病原性潜力.
- 区分真正的毒性因素和利基因素 (促进生存) 对工业细菌安全至关重要.
- 工业细菌,包括乳酸菌和葡萄球菌,需要严格的安全评估.
研究的目的:
- 评估已建立的对工业相关细菌物种的致病性评估方法.
- 用基因组和表型方法区分真正的毒性因素和利基因素.
- 评估工业细菌菌株的安全性,包括与临床分离物进行比较.
主要方法:
- 49个工业菌株和14个临床隔离物的基因组查与病毒毒性因子数据库 (VFDB) 相比.
- 欧洲食品安全局 (EFSA) 关于基因差异化值的应用.
- 表型分析包括细胞毒性,Caco-2细胞活力和血液溶解活性.
- 核心基因组分析,以评估工业和临床分离物之间的遗传学关系.
主要成果:
- 基因型评估显示,检查的工业菌株中没有真正的毒性因素.
- 根据核心基因组分析,临床分离物在遗传学上与工业菌株无法区分.
- 在表型分析中没有观察到细胞毒性,降低活力或血液溶解活性.
结论:
- 病毒毒性因子数据库 (VFDB) 选与欧洲食品安全局 (EFSA) 门有效地区分真正的病毒毒性因子和利基因素.
- 现型分析补充了基因型评估,但需要专家解释.
- 评估的工业细菌菌株被认为是安全的,缺乏显著的致病潜力.
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