野生类型和fli1b突变斑马鱼胚胎的单细胞RNA-seq数据
Luiza N Loges1, Ricardo DeMoya1, Valentina Laverde1
1Department of Pathology and Cell Biology, USF Heart Institute, 560 Channelside Drive Tampa, FL 33602, USA.
Data in brief
|February 2, 2026
概括
这项研究介绍了缺乏Fli1b转录因子的斑马鱼胚胎的单细胞RNA测序数据. 这些发现提供了关于血管和造血细胞发育的见解.
科学领域:
- 发展生物学 发展生物学
- 基因组学就是基因组学.
- 斑马鱼模型 斑马鱼模型
背景情况:
- Fli1b是一种ETS转录因子,对斑马鱼的血管和造血发育至关重要.
- 之前的研究已将Fli1b与这些关键的发育过程相关联.
研究的目的:
- 为了生成和分析来自野生类型和母细胞胚胎的单细胞RNA测序数据.
- 识别受Fli1b.缺失影响的细胞群和基因表达模式.
主要方法:
- 在受精后24小时,从大约40个野生类型和母性异位 (MZ) fli1b突变斑马鱼胚胎中制备了单细胞悬浮液.
- 用10X Genomics Chromium平台进行了RNA测序.
- 生物信息分析确定了34个不同的细胞群,通过标记基因表达进行注释.
主要成果:
- 野生类型和fli1b突变基因数据集的整合揭示了34个不同的细胞群.
- 细胞群根据特定标记物基因表达特征进行了注释.
- 这些数据提供了Fli1b函数的背景下早期斑马鱼发育的综合转录基因格局.
结论:
- 生成的单细胞RNA测序数据对于了解斑马鱼血管和造血发育的分子机制非常有价值.
- 这些转录组数据集可以用来研究斑马鱼和其他模型生物的更广泛的发育机制.
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