巴基斯坦婴儿的口腔微生物组概况以16S rRNA amplicon测序为特征
Muhammad Shahzad1,2, Muhammad Ismail2, Muhammad Junaid Ul Islam2
1Faculty of Dentistry, Zarqa University, Jordan.
Data in brief
|February 2, 2026
概括
这项研究揭示了巴基斯坦患有营养不良风险的婴儿口腔微生物组的发展. 关键的发现突出显示,在婴儿早期口腔细菌群体中,Bacillota,Streptococci和Veillonella占主导地位.
科学领域:
- 微生物学 微生物学
- 人类微生物组研究研究
- 婴儿健康 婴儿健康
背景情况:
- 口腔微生物组是一个复杂的生态系统,对健康至关重要.
- 关于易受伤害的婴儿群体,特别是那些有营养不良风险的婴儿群体的口腔微生物组发育的研究有限.
- 了解早期的口腔细菌殖民对于婴儿健康结果至关重要.
研究的目的:
- 调查来自营养不良流行地区的巴基斯坦婴儿口腔细菌社区的发展.
- 确定影响口腔微生物组组成的相关的母婴和环境因素.
- 为婴儿口腔微生物组发育的纵向研究提供基线数据.
主要方法:
- 从基线 (<28天) 的71名婴儿口腔抹布的纵向采样,并在3个月后跟踪65名婴儿口腔抹布.
- 使用DNBSEQ-G400平台进行DNA提取,PCR放大和16SrRNA放大序列测序.
- 生物信息学和统计分析使用Cutadapt,FLASH和R.
主要成果:
- 细菌群Bacillota (以前称为Firmicutes) 占主导地位 (87.6%在基线,84.3%在3个月).
- 链球菌 (基线66.9%,3个月55.4%) 和百花菌 (基线13.4%,3个月26.1%) 是主要的属性.
- 在136个样本中,有119个样本成功测序,提供了全面的口腔微生物组数据.
结论:
- 这项研究提供了对脆弱,营养不良的婴儿口腔细菌社区发展的首个全面见解.
- 这些发现为未来研究早期婴儿口腔微生物组动态奠定了基础.
- 测序数据在NCBI测序阅读档案 (PRJNA1303979) 中公开提供.
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