NIR-II生物模拟纳米平台光遗传学CD274编辑HNSCC免疫性用于增强光免疫疗法
Yang Chen1, Longcai Liu1, Xiaojuan Hu1
1Centre for Rehabilitation Medicine, Rehabilitation & Sports Medicine Research Institute of Zhejiang Province, Department of Rehabilitation Medicine, Cancer Centre, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China.
Materials today. Bio
|February 2, 2026
概括
研究人员开发了一种新的纳米平台,使用光线编辑头支状细胞癌 (HNSCC) 中的CD274基因. 这种方法通过改善T细胞透和减少瘤免疫性来增强免疫疗法.
科学领域:
- 生物医学工程 生物医学工程
- 癌症研究 癌症研究
- 免疫治疗是一种免疫疗法.
背景情况:
- 头部和部状细胞癌 (HNSCC) 具有较低的免疫性和有限的T细胞透,阻碍了免疫疗法的有效性.
- 目前的免疫疗法在克服HNSCC中免疫抑制瘤微环境方面面临挑战.
研究的目的:
- 开发一种使用近红外II (NIR-II) 光来编辑HNSCC中的CD274基因的新型光遗传策略.
- 通过改善T细胞透和克服低瘤免疫性来提高光免疫疗法的疗效.
主要方法:
- 一个仿生纳米平台 (ARPC) 使用红细胞膜进行工程设计,以提供NIR-II光热聚合物和CRISPR/Cas9等离子体.
- ARPC被静脉注射给携带HNSCC的小鼠,随后进行NIR-II激光照射以诱导热应激并通过CRISPR/Cas9.9触发CD274基因编辑.
- 光热效应还诱导了免疫细胞死亡,促进了CD8+ T细胞的透和增殖.
主要成果:
- 对ARPC的NIR-II辐射成功诱导热应激,上调Hsp70并触发CRISPR/Cas9介导的CD274基因编辑.
- 治疗有效地降低了HNSCC细胞中的CD274表达.
- 轻度光热疗法增强了CD8+T细胞的透和增殖,从而改善了光免疫疗法的结果.
结论:
- 开发的NIR-II光遗传CRISPR/Cas9策略有效地重编程HNSCC中的瘤微环境.
- 这种方法显示出克服低免疫性和改善HNSCC患者免疫治疗疗效的巨大潜力.
相关概念视频
02:23RNA Editing
9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
03:00Self-Evaluation: Self-Enhancement and Self-Verification
5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
01:16Bioavailability Enhancement: Drug Solubility Enhancement
260
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
260
01:27Bioavailability Enhancement: Drug Permeability Enhancement
205
Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
205
01:28CRISPR/Cas9 Genome Editing
1.9K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.9K
01:05Bioavailability Enhancement: Drug Stability Enhancement and GI Retention
218
Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
218