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一个协同的双模式生物传感器用于甲状腺酸盐检测,利用FRET和基于Ag NP的紫外线吸收
Qiongdan Zhang1, Qiongwei Teng1, Huihong Duan1
1TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine Changsha China wangwei402@hotmail.com libin@hnucm.edu.cn +86 15874100917 +86 13657438606.
RSC advances
|February 2, 2026
概括
一个新的双模式传感平台使用光和紫外线检测精确量化甲 (MTX). 这种方法提高了生物样本中治疗药物监测的可靠性.
科学领域:
- 分析化学 分析化学
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
背景情况:
- 准确量化甲醇 (MTX) 对于有效的治疗药物监测至关重要.
- 现有的MTX检测方法在复杂的生物基质中可能缺乏灵敏度或特异性.
- 需要开发新的传感平台来改善MTX监控.
研究的目的:
- 开发一种新的双模式传感平台 (DMSP),用于灵敏而准确的MTX量化.
- 整合光和紫外线检测模式,以提高可靠性和交叉验证.
- 评估平台在复杂的生物环境中的性能,例如血清样本.
主要方法:
- 开发了一个DMSP,将基于Förster共振能量转移 (FRET) 的光与以银纳米粒子 (AgNP) 增强的紫外线吸收相结合.
- 使用FAM作为光剂和BHQ作为光模式的灭剂.
- 采用AgNP进行405nm的紫外线检测,利用表面等离子体的共振特性.
主要成果:
- 实现了灵敏的MTX检测,具有良好的线性范围 (0.5-20μM).
- 在血清样本中显示的低检测极限 (光的0.56μM,紫外线的0.62μM).
- 对结构相似的分子表现出极好的特异性,证实了交叉验证能力.
结论:
- 开发的DMSP为MTX量化提供了强大而实用的方法.
- 双模式检测提高了治疗药物监测的准确性和可靠性.
- 该平台显示了复杂生物样本中临床应用的巨大潜力.
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