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从循环中丰富的组织特异性细胞外囊泡:探索液体活检的前景

Biancamaria Pierri1, Erez Eitan2, Kenneth W Witwer3

  • 1Department of Environmental Health Sciences, Mailman School of Public Health Columbia University New York USA.

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此摘要是机器生成的。

组织特异性的细胞外囊泡 (EVs) 对液体活检具有前景,但它们的异质性和各种检测方法带来了挑战. 改进验证和生物标志物识别对于推进转化医学中的EV应用至关重要.

关键词:
生物标志物 生物标志物细胞通信是细胞通信.细胞外囊泡中的细胞外囊泡.液体活检活检液体活检

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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 翻译医学是一种翻译医学.

背景情况:

  • 来自组织的细胞外囊泡 (EV) 在生物流体中循环,反映细胞状态并携带生物标志物.
  • 由于其信息分子载荷,EVs是进行非侵入性液体活检的有希望的途径.
  • 挑战包括EV异质性 (组成,起源) 和缺乏标准化的分离/表征方法.

研究的目的:

  • 审查有关组织特异性EV和它们在诊断中的潜力的当前知识.
  • 要突出阻碍基于EV的液体活检临床转化所面临的挑战.
  • 确定改善验证,可复制性和生物标志物发现的需求.

主要方法:

  • 关于组织特异性EVs现有研究的文献综述.
  • 对电动汽车目前的分离和表征技术的分析.
  • 识别电动汽车研究中的知识差距和局限性.

主要成果:

  • 组织特定的EV具有作为健康和疾病指标的巨大潜力.
  • 从复杂的生物流体样本中选择性地分离和验证EV存在重大障碍.
  • 目前用于EV分离和分析的方法往往缺乏标准化和可重复性.

结论:

  • 进一步的验证和可重复性研究对于开发可靠的组织特异性EV应用程序至关重要.
  • 发现强大的,组织丰富的生物标志物对于理解EV生理学和病理学至关重要.
  • 制定指导方针和标准化方法将有助于将EV液体活检纳入翻译医学.