液体剪切应激对人类四肢上皮细胞的影响
Sophia Masterton1,2, Mark Ahearne1,2
1Department of Mechanical, Manufacturing and Biomedical Engineering, School of Engineering, Trinity College Dublin, University of Dublin, Ireland.
流体剪切应力显著改变边缘上皮细胞 (LEC) 行为和表型,观察到TRPV4通道激活和分层. 这些细胞对机械应激的反应显著依赖于供体,突出显示角膜表面维护的个体变化.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 边缘上皮细胞 (LECs) 通过迁移和替换维持角膜表面.
- 在LEC中,闪和撕裂膜运动造成的剪切压力.
- 剪切应力对LEC行为的影响尚不清楚.
研究的目的:
- 为了研究流体剪切应力对LEC行为的影响.
- 分析LEC对剪切应力的供体特异反应.
主要方法:
- 使用商业流体流系统 (ibidi) 应用受控剪切应力.
- 在低,高和无流条件下检查了来自三个不同捐赠者的LEC.
- 评估细胞表型,分层,TRPV4激活,粘附和屏障功能.
主要成果:
- 剪切应激诱导了LEC表型的变化,在捐赠者之间有显著的差异.
- 在所有暴露于剪切作用的LEC中,TRPV4 (过渡受体潜在化物4) 表达被上调.
- 细胞分层仅在受到剪切应力的影响的LEC中观察到.
结论:
- 剪切应力是调节LEC行为的一个关键因素.
- 捐赠者对捐赠者的变异性和细胞群异质性是LEC研究中的重要考虑因素.
- 了解剪切应力效应对于角膜表面研究和治疗至关重要.
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